Competitive ELISA
The Corticosterone ELISA quantitates coritcosterone in serum, plasma, urine, dried fecal extracts or cell culture medium. The assay will exclusively recognize both natural and recombinant coritcosterone. Principle of the method The corticosterone solid-phase sandwich ELISA (enzyme-linked immunosorbent assay) is designed to measure the amount of the target bound between a matched antibody pair. A target-specific antibody has been pre-coated in the wells of the supplied microplate. Samples, standards, or controls are then added into these wells and bind to the immobilized (capture) antibody. The sandwich is formed by the addition of the second (detector) antibody, binding to the target on a different epitope from the capture antibody. A conjugated enzyme has been incorporated into the assay. After incubation and washing steps to rid the microplate of unbound substances, a substrate solution is added that reacts with the enzyme-antibody-target complex to produce measurable signal. The intensity of this signal is inversely proportional to the concentration of target present in the original specimen. Rigorous validation Each manufactured lot of this ELISA kit is quality tested for criteria such as sensitivity, specificity, precision, and lot-to-lot consistency. See manual for more information on validation.
CORTICOSTERONE is an adrenocortical steroid that has modest but significant activities as a mineralocorticoid and a glucocorticoid. [National Center for Biotechnology Information. PubChem Compound Database; CID=5753].
| Marque | Invitrogen |
|---|---|
| Réf. B-LABO | BL-RE-090416 |
| Réf. fabricant | 17845117 |
| Plage de dosage | 78,125-10,000 pg/ml |
| Sensibilité du dosage | 18,6 pg/ml |
| Doser | Dosage multiplex sur la plateforme Luminex |
| Conjugué | HRP |
| Méthode de détection | Fluorescent |
| Type de produit | Kit ELISA |
| Type d’échantillon | Extrait de matières fécales, Sérum, Surnageant, Surnageant, Urine |
| Suffisant pour | 10 x 96 dosages |
| Espèces cibles | Humaine |
| À utiliser avec (application) | ELISA, dosages de protéines, tests d’activité, biologie des protéines, biologie de la reproduction, inflammation, stress oxydatif, métabolisme, signalisation cellulaire, épigénétique, hématologie, dommages à l’ADN, biologie du cancer, immunologie, neurosciences, neurobiologie, détection d’hormones stéroïdes |
| À utiliser avec (équipement) | Lecteur de microplaques colorimétriques |
| Coefficient de variation inter-dosage | 7,9% |
| Coefficient de variation intra-dosage | 5,2% |
| Contenus des kits | Plaque à 96 puits pré-enduite, Étalon, Concentrat de diluant de dosage, Anticorps de détection conjugué au HRP, Tampon de lavage, Chromogène, Solution d’arrêt, Couvercles de plaques adhésives |
| Marqueur ou colorant | HRP |
| État réglementaire | RUO |
| Disciplines de recherche | Inflammation |
| Volume d’échantillon | Extrait fécal, 50 μl ; plasma, 1 μl ; sérum, 1 μl ; surnageant, 50 μl ; urine, 2,5 μl |
| Conditions de stockage | -20°C |
| Cible | Produits chimiques |
| Heure du test | 1 h 20 min |
| Durée totale du dosage | 1 h 30 min |
