Competitive ELISA
The Dehydroepiandrosterone Sulfate (DHEA-S) ELISA quantitates DHEA-Se in serum,plasma,urine,saliva,dried fecal extracts or cell culture medium.
The Dehydroepiandrosterone sulfate (DHEA-S) Competitive ELISA research-use-only kit uses a specifically generated antibody to measure Dehydroepiandrosterone sulfate (DHEA-S) in serum,plasma,urine,and saliva samples,and in fecal extracts. The kit will also quantitatively measure DHEA-S present in tissue culture media samples. A DHEA-S standard is provided to generate a standard curve for the assay and all samples should be read off the standard curve. Standards or diluted samples are pipetted into a clear microtiter plate coated with an antibody to capture sheep antibodies. A DHEA-S-peroxidase conjugate is added to the standards and samples in the wells. The binding reaction is initiated by the addition of a polyclonal antibody to DHEA-S to each well. After a 2-hour incubation the plate is washed and substrate is added. The substrate reacts with the bound DHEA-S-peroxidase conjugate. After a short incubation,the reaction is stopped and the intensity of the generated color is detected in a microtiter plate reader capable of measuring at 450 nm.
Each manufactured lot of this ELISA kit is quality tested for criteria such as sensitivity,specificity,precision,and lot-to-lot consistency. See manual for more information on validation.
Dehydroepiandrosterone sulfate (DHEA-S) is the major C19 steroid secreted by the adrenal cortex and a precursor in testosterone and estrogen biosynthesis. DHEA-S is identical across all species and so this kit should measure DHEA-S from all sources. It is produced by the addition of a sulfate group to dehydroepiandrosterone (DHEA), catalyzed by the sulfotransferase enzymes, SULT1A1 and SULT1E1, which also produce estrone sulfate from estrone. DHEA sulfate can also be back-converted to DHEA through the action of steroid sulfatase.
| Marque | Invitrogen |
|---|---|
| Réf. B-LABO | BL-RE-090532 |
| Réf. fabricant | 17895207 |
| Plage de dosage | 96-60,000 pg/ml |
| Sensibilité du dosage | 90,9 pg/ml |
| Doser | Dosage multiplex sur la plateforme Luminex |
| Conjugué | HRP |
| Méthode de détection | Fluorescent |
| Type de produit | Kit ELISA |
| Type d’échantillon | Extraits fécaux, plasma, salive, sérum, surnageant, Urine |
| Suffisant pour | 10 x 96 dosages |
| Espèces cibles | Humaine |
| À utiliser avec (application) | ELISA, dosages de protéines, tests d’activité, biologie des protéines, biologie de la reproduction, inflammation, stress oxydatif, métabolisme, signalisation cellulaire, épigénétique, hématologie, dommages à l’ADN, biologie du cancer, immunologie, neurosciences, neurobiologie, détection d’hormones stéroïdes |
| À utiliser avec (équipement) | Lecteur de microplaques colorimétriques |
| Coefficient de variation inter-dosage | 8,4% |
| Coefficient de variation intra-dosage | 7% |
| Contenus des kits | Plaque à 96 puits pré-enduite, Étalon, Concentrat de diluant de dosage, Anticorps de détection conjugué au HRP, Tampon de lavage, Chromogène, Solution d’arrêt, Couvercles de plaques adhésives |
| Marqueur ou colorant | HRP |
| État réglementaire | RUO |
| Disciplines de recherche | Biologie de la reproduction |
| Volume d’échantillon | Extrait fécal, 50 μl ; plasma, 1 μl ; salive, 25 μl ; sérum, 1 μl ; surnageant, 50 μl ; urine, 25 μl |
| Conditions de stockage | -20°C |
| Cible | Produits chimiques |
| Heure du test | 1 h 20 min |
| Durée totale du dosage | 2 h 30 min |
