Competitive ELISA
The Levonorgestrel (LNG) ELISA quantitates LNG in serum,plasma,urine,dried fecal extracts,saliva,milk,or cell culture medium.
The Levonorgestrel (LNG) Competitive ELISA research-use-only kit is designed to quantitatively measure levonorgestrel present in serum,plasma,saliva,urine,dried fecal samples,milk,and tissue culture media samples. This kit measures total LNG in extracted serum,plasma,milk and fecal samples. A levonorgestrel stock solution is provided to generate a standard curve for the assay and all samples should be read off the standard curve. Standards or diluted samples are pipetted into a clear microtiter plate coated with an antibody to capture rabbit antibodies. A levonorgestrel-peroxidase conjugate is added to the standards and samples in the wells. The binding reaction is initiated by the addition of a rabbit polyclonal antibody to levonorgestrel to each well. After a 1-hour incubation the plate is washed and substrate is added. The substrate reacts with the bound levonorgestrel-peroxidase conjugate. After a short incubation,the reaction is stopped and the intensity of the generated color is detected in a microtiter plate reader capable of measuring at 450 nm.
Each manufactured lot of this ELISA kit is quality tested for criteria such as sensitivity,specificity,precision,and lot-to-lot consistency. See manual for more information on validation.
Levonorgestrel (4-Estren-17alpha-Ethynyl-18-homo-17beta-ol-3-one (LNG) is a synthetic steroid analogue.
| Marque | Invitrogen |
|---|---|
| Réf. B-LABO | BL-RE-090535 |
| Réf. fabricant | 17825217 |
| Plage de dosage | 6,25-200 pg/ml |
| Sensibilité du dosage | 2,2 pg/ml |
| Doser | Dosage multiplex sur la plateforme Luminex |
| Conjugué | HRP |
| Méthode de détection | Fluorescent |
| Type de produit | Kit ELISA |
| Type d’échantillon | Extrait fécal, Lait de vache, Plasma, Salive, Sérum, Surnageant, Urine, Eau |
| Suffisant pour | 10 x 96 dosages |
| Espèces cibles | Humaine |
| À utiliser avec (application) | ELISA, dosages de protéines, tests d’activité, biologie des protéines, biologie de la reproduction, inflammation, stress oxydatif, métabolisme, signalisation cellulaire, épigénétique, hématologie, dommages à l’ADN, biologie du cancer, immunologie, neurosciences, neurobiologie, détection d’hormones stéroïdes |
| À utiliser avec (équipement) | Lecteur de microplaques colorimétriques |
| Coefficient de variation inter-dosage | 9,1% |
| Coefficient de variation intra-dosage | 9% |
| Contenus des kits | Plaque à 96 puits pré-enduite, Étalon, Concentrat de diluant de dosage, Anticorps de détection conjugué au HRP, Tampon de lavage, Chromogène, Solution d’arrêt, Couvercles de plaques adhésives |
| Marqueur ou colorant | HRP |
| État réglementaire | RUO |
| Disciplines de recherche | Biologie de la reproduction |
| Volume d’échantillon | Extrait fécal, 100 μl ; lait, 100 μl ; plasma, 100 μl ; salive, 50 μl ; sérum, 100 μl ; surnageant, 100 μl ; urine, 25 μl ; eau,100 μl |
| Conditions de stockage | -20°C |
| Cible | Produits chimiques |
| Heure du test | 1 h 20 min |
| Durée totale du dosage | 1 h 30 min |
