The Aspartate Aminotransferase (AST/GOT) Assay Kit is a highly reliable and sensitive assay designed for the quantitative measurement of aspartate aminotransferase activity in various sample types. AST/GOT is a key enzyme in nitrogen metabolism, which is widely found in plasma and body tissues, including liver, heart, skeletal muscle, kidney, brain, pancreas, lung, and erythrocytes. Changes in AST/GOT activity were found in acute pancreatitis, ischemic stroke, severe burns, periodontitis, acute renal disease, and motor neuron disease. The assay is based on the ability of AST/GOT to enable alpha-ketoglutaric acid and aspartic acid to displace amino and keto groups to form glutamic acid and oxaloacetic acid. Oxaloacetic acid can decarboxylate itself to form pyroracemic acid during the reaction. Pyroracemic acid reacts with 2,4-dinitro phenyl hydrazine (DNPH) to form 2,4, dinitrophenylhydrazone, showing reddish-brown color in alkaline solution. The OD values can be measured to calculate the enzyme activity. Key features • Simple and fast protocol—user-friendly, requiring minimal hands-on time and providing rapid results within a short incubation period • Broad range of kits—across key analytes and targets within the most important metabolic pathways • Consistent—strict quality control criteria to ensure reproducible and reliable results
Caractéristiques techniques
| Marque | Invitrogen |
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| Réf. B-LABO | BL-RE-090648 |
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| Réf. fabricant | 17992091 |
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| Plage de dosage | 1.1–72.3 IU/L |
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| Sensibilité du dosage | 1.1 IU/L |
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| Contenu et stockage | Store at 2–8°C.<br><br> |
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| Méthode de détection | Colorimetric |
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| Type d’échantillon | Cell Culture Supernatant, Plasma, Serum, Tissue Homogenates |
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| À utiliser avec (équipement) | Microplate Reader |
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| Temps d’incubation | 50 min. |
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| Coefficient de variation inter-dosage | 6.8 |
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| Coefficient de variation intra-dosage | 5.3 |
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| Volume d’échantillon | 10 μL |
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| Conditions d’expédition | Dry Ice, 2–8°C |
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| Kit cible nommé | Aspartate Aminotransferase (AST/GOT) |
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| Durée totale du dosage | 80 min. |
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