These complete, ready-to-use kits include clear 96-well plate(s), other assay specific reagents, buffers, and a lot-specific technical data sheet.
The Cell Total Iron Colorimetric Assay Kit is a highly reliable and sensitive assay designed for the quantitative measurement of total iron content in cells. Iron plays an important role in various physiological functions. The ferrous ion is a key element in heme and hemoglobin and is present in many biochemical reactions. In this assay iron ions in samples can be reduced into ferrous ions (Fe2+) when subjected to reductants. The latter then bind to probes in solution to form complexes that have a maximum absorption peak at 593 nm. The concentration of iron can be indirectly calculated by measuring the OD value. Key features • Simple and fast protocol—user-friendly, requiring minimal hands-on time and providing rapid results within a short incubation period • Broad range of kits—across key analytes and targets within the most important metabolic pathways • Consistent—strict quality control criteria to ensure reproducible and reliable results
| Marque | Invitrogen |
|---|---|
| Réf. B-LABO | BL-RE-090653 |
| Réf. fabricant | 17952011 |
| Plage de dosage | 0.4–50 μmol/L |
| Sensibilité du dosage | 0.4 μmol/L |
| Contenu et stockage | Store at 2–8°C.<br><br> |
| Méthode de détection | Colorimetric |
| Type d’échantillon | Whole Cells |
| À utiliser avec (équipement) | Microplate Reader |
| Temps d’incubation | 40 min. |
| Coefficient de variation inter-dosage | 1.5 |
| Coefficient de variation intra-dosage | 1.3 |
| Volume d’échantillon | 80 μL |
| Conditions d’expédition | Dry Ice, 2–8°C |
| Kit cible nommé | Cell Total Iron |
| Durée totale du dosage | 80 min. |
