Cortisol is the most potent glucocorticoid produced by the human adrenal. It is synthesized from cholesterol and its production is stimulated by pituitary adrenocorticotropic hormone (ACTH) which is regulated by corticotropin releasing factor (CRF). ACTH and CRF secretions are inhibited by high cortisol levels in a negative feedback loop. In plasma a majority of cortisol is bound with high affinity to corticosteroid binding globulin (CBG or transcotin). Cortisol acts through specific intracellular receptors and affects numerous physiologic systems including immune function, glucose counter regulation, vascular tone, and bone metabolism.
Caractéristiques techniques
| Marque | Invitrogen |
| Réf. B-LABO | BL-RE-090716 |
| Réf. fabricant | 18057484 |
| Plage de dosage | 1.56 to 100 ng/mL |
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| Sensibilité du dosage | 0.96 ng/mL |
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| Conjugué | HRP |
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| Type de produit | ELISA |
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| Type d’échantillon | Serum |
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| À utiliser avec (équipement) | Colorimetric Microplate Reader |
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| Coefficient de variation inter-dosage | <10% |
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| Coefficient de variation intra-dosage | <10% |
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| Contenus des kits | Pre-coated 96 well plate, Standard, Biotinylated Detection Antibody, HRP Conjugate, Standard & Sample Diluent, Biotinylated Detection Antibody Diluent, HRP Conjugate Diluent, Wash Buffer, TMB Substrate, Stop Solution, Plate Sealer |
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| Marqueur ou colorant | HRP |
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| Quantité | 96 Tests |
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| État réglementaire | RUO |
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| Volume d’échantillon | 50 µL |
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| Conditions de stockage | Refer to product documentation for component specific storage temperature. |
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| Cible | Bovine |
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| Heure du test | 1 hr 20 min |
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| Durée totale du dosage | 3 hr 30 min |
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