For the quantitative detection of Human Survivin
Survivin (IAP4) is the smallest member of the Inhibitors of Apoptosis Protein (IAP) gene family. The IAPs are involved in multiple cell functions such as cell signaling, cell division, metabolism, and exhibits differential expression in nearly all human cancers, but not in most normal tissues. IAP family members usually contain multiple baculovirus IAP repeat (BIR) domains, but the Survivin gene encodes proteins with only a single BIR domain. The encoded proteins also lack a C-terminus RING finger domain. Gene expression is high during fetal development and in most tumors yet low in adult tissues. Antisense transcripts are involved in the regulation of survivin’s gene expression. Survivin is expressed in the G2/M phase of the cell cycle in a cycle-regulated manner. At the beginning of mitosis, survivin associates with microtubules of the mitotic spindle in a specific and saturable reaction that is regulated by microtubule dynamics. Disruption of survivin-microtubule interactions results in loss of survivin's anti-apoptosis function and increased caspase-3 activity, a mechanism involved in cell death, during mitosis. Survivin may counteract a default induction of apoptosis in G2/M phase. Survivin is also abundantly expressed in brain tissues (astrocytes and some neurons) of adult rats following traumatic brain injury. Survivin has been found co-expressed with NeuN (mature neuronal marker) and PCNA (a cell cycle protein). Survivin might affect regulation of neural cell proliferative responses after brain injury. At least four transcript variants encoding distinct isoforms have been found for this gene, but the full-length natures of only three of them have been determined. The overexpression of survivin in cancer may overcome this apoptotic checkpoint and favor aberrant progression of transformed cells through mitosis.
| Marque | Invitrogen |
|---|---|
| Réf. B-LABO | BL-RE-090855 |
| Réf. fabricant | 18027394 |
| Numéro d’adhésion | O15392 |
| Plage de dosage | 31.25 to 2000 pg/mL |
| Sensibilité du dosage | 18.75 pg/mL |
| Conjugué | HRP |
| Type de produit | ELISA |
| Type d’échantillon | Serum |
| À utiliser avec (équipement) | Colorimetric Microplate Reader |
| Identification génétique (Entrez) | 332 |
| Symbole de gène(s) | Birc5 |
| Coefficient de variation inter-dosage | <10% |
| Coefficient de variation intra-dosage | <10% |
| Contenus des kits | Pre-coated 96 well plate, Standard, Biotinylated Detection Antibody, HRP Conjugate, Standard & Sample Diluent, Biotinylated Detection Antibody Diluent, HRP Conjugate Diluent, Wash Buffer, TMB Substrate, Stop Solution, Plate Sealer |
| Marqueur ou colorant | HRP |
| Quantité | 96 Tests |
| État réglementaire | RUO |
| Volume d’échantillon | 100 µL |
| Conditions de stockage | Refer to product documentation for component specific storage temperature. |
| Cible | Human |
| Heure du test | 1 hr 20 min |
| Durée totale du dosage | 3 hr 30 min |
