The Reactive Oxygen Species (ROS) Fluorometric Assay Kit is a highly reliable and sensitive assay designed for the quantitative measurement of ROS in fresh tissue and live cell samples. ROS are highly reactive molecules that play a crucial role in various physiological and pathological processes, including oxidative stress, inflammation, and cellular signaling. The assay works by utilizing the fluorescent probe 2',7'-dichlorodihydrofluorescein diacetate (DCFH-DA) to detect the presence of ROS. DCFH-DA is taken up by cells and is converted to dichlorofluorescein (DCFH) by intracellular esterase. DCFH is oxidized by ROS to the strong fluorescent DCF. The fluorescence of DCF (Ex/Em 502/525 nm) is directly proportional to the amount of ROS present in the sample. Key features • Simple and fast protocol—user-friendly, requiring minimal hands-on time and providing rapid results within a short incubation period • Broad range of kits—across key analytes and targets within the most important metabolic pathways • Consistent—strict quality control criteria to ensure reproducible and reliable results
Caractéristiques techniques
| Marque | Invitrogen |
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| Réf. B-LABO | BL-RE-091039 |
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| Réf. fabricant | 17932171 |
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| Contenu et stockage | Reagent 1: –20°C<br>Reagent 2: 2–8°C<br><br> |
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| Méthode de détection | Fluorescence |
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| Type d’échantillon | Cell Culture, Live Tissue |
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| À utiliser avec (équipement) | Spectrophotometer, Microplate Reader, Flow Cytometer |
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| Temps d’incubation | 30-60 min. |
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| Conditions d’expédition | Dry Ice, 2–8°C |
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| Kit cible nommé | Reactive Oxygen Species (ROS) |
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| Durée totale du dosage | 60 min. |
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