Sandwich ELISA Kit
The Mouse Immunoglobulin A (Ms IgA) ELISA quantitates Ms IgA in mouse serum, plasma, or cell culture medium. The assay will exclusively recognize both natural and recombinant Ms IgA. Principle of the method The Mouse IgA solid-phase sandwich ELISA (enzyme-linked immunosorbent assay) is designed to measure the amount of the target bound between a matched antibody pair. A target-specific antibody has been pre-coated in the wells of the supplied microplate. Samples, standards, or controls are then added into these wells and bind to the immobilized (capture) antibody. The sandwich is formed by the addition of the second (detector) antibody, a substrate solution is added that reacts with the enzyme-antibody-target complex to produce measurable signal. The intensity of this signal is directly proportional to the concentration of target present in the original specimen. Rigorous validation Each manufactured lot of this ELISA kit is quality tested for criteria such as sensitivity, specificity, precision, and lot-to-lot consistency. See manual for more information on validation.
IgA, which is mainly found as a dimer through association of the J chain, is found in mucous secretions, but not in plasma.
| Marque | Invitrogen |
|---|---|
| Réf. B-LABO | BL-RE-091113 |
| Réf. fabricant | 18527824 |
| Plage de dosage | 2.05 to 500 ng/mL |
| Sensibilité du dosage | 1.5 ng/mL |
| Plage d’étalonnage | 1.5 ng/mL to 500 ng/mL |
| Doser | Solid-phase sandwich ELISA |
| Conjugué | Biotin |
| Type de produit | ELISA Kit |
| Famille de protéines | Other Proteins |
| Type d’échantillon | Plasma |
| Spécificité | This ELISA pair antibody detects mouse IgA. Other species not determined. |
| Suffisant pour | 96 assays |
| Espèces cibles | Mouse |
| À utiliser avec (application) | ELISA, Protein Assays, Protein Biology, Cancer Biology, Immunology, Neuroscience, Neurobiology, Protein Detection, Protein Analysis |
| À utiliser avec (équipement) | Colorimetric Microplate Reader |
| Coefficient de variation inter-dosage | <12% |
| Coefficient de variation intra-dosage | <10% |
| Contenus des kits | Pre-coated 96 well plate |
| Marqueur ou colorant | HRP |
| Quantité | 10 x 96 Tests |
| État réglementaire | RUO |
| Disciplines de recherche | Immunology |
| Volume d’échantillon | Plasma, 2 µL; Serum, 2 µL; Supernatant, 100 µL |
| Conditions de stockage | 2°C to 8°C |
| Cible | Mouse |
| Heure du test | 1 hr. 20 min. |
| Durée totale du dosage | 4 hr. 45 min. |
