Sandwich ELISA Kit
The Mouse Hepatocyte Growth Factor(Ms HGF) Cell Lysates ELISA quantitates Ms HGF in mouse cell or tissue lysates. The assay will exclusively recognize both natural and recombinant Ms HGF. Principle of the method The Mouse HGF solid-phase sandwich ELISA (enzyme-linked immunosorbent assay) is designed to measure the amount of the target bound between a matched antibody pair. A target-specific antibody has been pre-coated in the wells of the supplied microplate. Samples, standards, or controls are then added into these wells and bind to the immobilized (capture) antibody. The sandwich is formed by the addition of the second (detector) antibody, a substrate solution is added that reacts with the enzyme-antibody-target complex to produce measurable signal. The intensity of this signal is directly proportional to the concentration of target present in the original specimen. Rigorous validation Each manufactured lot of this ELISA kit is quality tested for criteria such as sensitivity, specificity, precision, and lot-to-lot consistency. See manual for more information on validation.
Hepatocyte growth factor (HGF) is a member of the plasminogen subfamily of S1 peptidases. The inactive polypeptide formed upon translation undergoes cleavage to produce a 69 kDa alpha-chain and 34 kDa beta-chain. A disulphide bond between the two chains makes active HGF. HGF is secreted by mesenchymal cells and stimulates mitogenesis, cell motility, and matrix invasion. It is involved in cellular growth and morphogenesis in a wide variety of tissue types. In humans, HGF is present on chromosome 7.
| Marque | Invitrogen |
|---|---|
| Réf. B-LABO | BL-RE-091121 |
| Réf. fabricant | 18597874 |
| Numéro d’adhésion | Q08048 |
| Plage de dosage | 0.614 to 60 ng/mL |
| Sensibilité du dosage | 400 pg/mL |
| Plage d’étalonnage | 400 pg/mL to 60 ng/mL |
| Doser | Solid-phase sandwich ELISA |
| Conjugué | Biotin |
| Type de produit | ELISA Kit |
| Famille de protéines | Chemokine & Receptors |
| Type d’échantillon | Cell Lysates |
| Spécificité | The antibody pair provided in this kit recognizes mouse HGF. |
| Suffisant pour | 96 assays |
| Espèces cibles | Mouse |
| À utiliser avec (application) | ELISA, Protein Assays, Protein Biology, Cancer Biology, Immunology, Neuroscience, Neurobiology, Protein Detection, Protein Analysis |
| À utiliser avec (équipement) | Colorimetric Microplate Reader |
| Identification génétique (Entrez) | 15234 |
| Symbole de gène(s) | C230052L06Rik,HGF/SF,NK1,NK2,SF,SF/HGF |
| Coefficient de variation inter-dosage | <12% |
| Coefficient de variation intra-dosage | <10% |
| Contenus des kits | Biotinylated Detection Antibody |
| Marqueur ou colorant | HRP |
| Quantité | 10 x 96 Tests |
| État réglementaire | RUO |
| Disciplines de recherche | Apoptosis |
| Volume d’échantillon | Cell Lysate,50 µL |
| Conditions de stockage | 2°C to 8°C |
| Synonyme | C230052L06Rik, HGF/SF, NK1, NK2, SF, SF/HGF |
| Cible | Mouse |
| Heure du test | 1 hr. 20 min. |
| Durée totale du dosage | 4 hr. 45 min. |
