The Total Antioxidant Capacity (T-AOC) Colorimetric Assay Kit is a highly reliable and sensitive assay designed for the quantitative measurement of T-AOC in serum, plasma, whole blood, tissue, cells, and culture supernatant. T-AOC refers to the overall ability of a biological sample to counteract oxidative stress by neutralizing reactive oxygen species (ROS) and other harmful free radicals. It represents the cumulative effect of both enzymatic and non-enzymatic antioxidants present in the sample. The assay quantifies the ability of the sample to reduce ferric ions (Fe3+) to ferrous ions (Fe2+) using a phenanthroline substance. The resulting complex exhibits a characteristic absorbance at 520 nm, which is directly proportional to the T-AOC of the sample. Key features • Simple and fast protocol—user-friendly, requiring minimal hands-on time and providing rapid results within a short incubation period • Broad range of kits—across key analytes and targets within the most important metabolic pathways • Consistent—strict quality control criteria to ensure reproducible and reliable results
Caractéristiques techniques
| Marque | Invitrogen |
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| Réf. B-LABO | BL-RE-091579 |
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| Réf. fabricant | 17992141 |
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| Plage de dosage | 0.62–190.43 U/mL |
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| Sensibilité du dosage | 0.62 U/mL |
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| Contenu et stockage | Store at 2–8°C.<br><br> |
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| Méthode de détection | Colorimetric |
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| Type d’échantillon | Cell Culture Supernatant, Plasma, Serum, Tissue Homogenates, Whole Blood, Whole Cells |
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| À utiliser avec (équipement) | Microplate Reader |
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| Temps d’incubation | 40 min. |
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| Coefficient de variation inter-dosage | 5.6 |
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| Coefficient de variation intra-dosage | 4.8 |
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| Volume d’échantillon | 20 μL |
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| Conditions d’expédition | Dry Ice, 2–8°C |
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| Kit cible nommé | Total Antioxidant Capacity (T-AOC) |
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| Durée totale du dosage | 50 min. |
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