Sandwich ELISA Kit
The Endothelin-1 (ET-1) Multispecies ELISA quantitates Endothelin-1 in human, bovine, procine, dog, rat, and mouse serum, plasma, urine, or cell culture medium. The assay will exclusively recognize both natural and recombinant Endothelin-1. Principle of the method The Endothelin-1 solid-phase sandwich ELISA (enzyme-linked immunosorbent assay) is designed to measure the amount of the target bound between a matched antibody pair. A target-specific antibody has been pre-coated in the wells of the supplied microplate. Samples, standards, or controls are then added into these wells and bind to the immobilized (capture) antibody. The sandwich is formed by the addition of the second (detector) antibody, a substrate solution is added that reacts with the enzyme-antibody-target complex to produce measurable signal. The intensity of this signal is inversely proportional to the concentration of target present in the original specimen. Rigorous validation Each manufactured lot of this ELISA kit is quality tested for criteria such as sensitivity, specificity, precision, and lot-to-lot consistency. See manual for more information on validation.
Endothelins (ET) show potent constrictor activity in vascular and non-vascular smooth muscle. This family of 21-amino acid peptides exists in at least three isoforms - ET-1, ET-2, and ET-3, and is produced in endothelial and epithelial cells. ET's can mediate biological effects in cells and tissues, and have been shown to bind to an ET receptor in the lung, kidney, heart, and liver.
| Marque | Invitrogen |
|---|---|
| Réf. B-LABO | BL-RE-091648 |
| Réf. fabricant | 17855117 |
| Numéro d’adhésion | P05305 |
| Plage de dosage | 0,781 à 100 pg/ml |
| Sensibilité du dosage | 0,579 pg/ml |
| Doser | Dosage multiplex sur la plateforme Luminex |
| Conjugué | HRP |
| Méthode de détection | Fluorescent |
| Type de produit | Kit ELISA |
| Type d’échantillon | Plasma, Sérum, Surnageant, Urine |
| Suffisant pour | 10 x 96 dosages |
| Espèces cibles | Humaine |
| À utiliser avec (application) | ELISA, dosages de protéines, tests d’activité, biologie des protéines, biologie de la reproduction, inflammation, stress oxydatif, métabolisme, signalisation cellulaire, épigénétique, hématologie, dommages à l’ADN, biologie du cancer, immunologie, neurosciences, neurobiologie, détection d’hormones stéroïdes |
| À utiliser avec (équipement) | Lecteur de microplaques colorimétriques |
| Identification génétique (Entrez) | 1906 |
| Symbole de gène(s) | EDN1 |
| Coefficient de variation inter-dosage | 3,7% |
| Coefficient de variation intra-dosage | 3,3% |
| Contenus des kits | Plaque à 96 puits pré-enduite, Étalon, Concentrat de diluant de dosage, Anticorps de détection conjugué au HRP, Tampon de lavage, Chromogène, Solution d’arrêt, Couvercles de plaques adhésives |
| Marqueur ou colorant | HRP |
| État réglementaire | RUO |
| Disciplines de recherche | Signalisation cellulaire |
| Volume d’échantillon | Plasma, 50 μl ; Sérum, 50 μl ; Surnageant, 2,5 μl ; Urine, 50 μl |
| Conditions de stockage | -20°C |
| Cible | Humaine |
| Heure du test | 1 heures 20 min. |
| Durée totale du dosage | 2 heures 30 min. |
