Sandwich ELISA Kit
The Mouse Endocan (Ms ESM1) ELISA quantitates Ms Endocan in mouse serum, plasma, or cell culture medium. The assay will exclusively recognize both natural and recombinant Ms Endocan. Principle of the method The Mouse Endocan solid-phase sandwich ELISA (enzyme-linked immunosorbent assay) is designed to measure the amount of the target bound between a matched antibody pair. A target-specific antibody has been pre-coated in the wells of the supplied microplate. Samples, standards, or controls are then added into these wells and bind to the immobilized (capture) antibody. The sandwich is formed by the addition of the second (detector) antibody, a substrate solution is added that reacts with the enzyme-antibody-target complex to produce measurable signal. The intensity of this signal is directly proportional to the concentration of target present in the original specimen. Rigorous validation Each manufactured lot of this ELISA kit is quality tested for criteria such as sensitivity, specificity, precision, and lot-to-lot consistency. See manual for more information on validation.
This gene encodes a secreted protein which is mainly expressed in the endothelial cells in human lung and kidney tissues. The expression of this gene is regulated by cytokines, suggesting that it may play a role in endothelium-dependent pathological disorders. The transcript contains multiple polyadenylation and mRNA instability signals. Two transcript variants encoding different isoforms have been found for this gene.
| Marque | Invitrogen |
|---|---|
| Réf. B-LABO | BL-RE-091719 |
| Réf. fabricant | 18537924 |
| Numéro d’adhésion | Q9QYY7 |
| Plage de dosage | 0.123 to 30 ng/mL |
| Sensibilité du dosage | 0.1 ng/mL |
| Plage d’étalonnage | 0.1 ng/mL to 30 ng/mL |
| Doser | Solid-phase sandwich ELISA |
| Conjugué | Biotin |
| Type de produit | ELISA Kit |
| Famille de protéines | Growth Factors & Receptors |
| Type d’échantillon | Plasma |
| Spécificité | This ELISA antibody pair detects mouse Endocan, it shows no significant cross-reactivity with recombinant human Endocan. |
| Suffisant pour | 96 assays |
| Espèces cibles | Mouse |
| À utiliser avec (application) | ELISA, Protein Assays, Protein Biology, Cancer Biology, Immunology, Neuroscience, Neurobiology, Protein Detection, Protein Analysis |
| À utiliser avec (équipement) | Colorimetric Microplate Reader |
| Identification génétique (Entrez) | 71690 |
| Symbole de gène(s) | 0610042H23Rik,AV004503,ESM-1 |
| Coefficient de variation inter-dosage | <12% |
| Coefficient de variation intra-dosage | <10% |
| Contenus des kits | Pre-coated 96 well plate |
| Marqueur ou colorant | HRP |
| Quantité | 10 x 96 Tests |
| État réglementaire | RUO |
| Disciplines de recherche | Development |
| Volume d’échantillon | Plasma, 50 µL; Serum, 50 µL; Supernatant, 100 µL |
| Conditions de stockage | 2°C to 8°C |
| Synonyme | 0610042H23Rik, AV004503, ESM-1 |
| Cible | Mouse |
| Heure du test | 1 hr. 20 min. |
| Durée totale du dosage | 4 hr. 45 min. |
