Sandwich ELISA Kit
The Human CKMB ELISA quantitates Hu CKMB in human serum, plasma, or cell culture medium. The assay will exclusively recognize both natural and recombinant Hu CKMB. Principle of the method The Human CKMB solid-phase sandwich ELISA (enzyme-linked immunosorbent assay) is designed to measure the amount of the target bound between a matched antibody pair. A target-specific antibody has been pre-coated in the wells of the supplied microplate. Samples, standards, or controls are then added into these wells and bind to the immobilized (capture) antibody. The sandwich is formed by the addition of the second (detector) antibody, a substrate solution is added that reacts with the enzyme-antibody-target complex to produce measurable signal. The intensity of this signal is directly proportional to the concentration of target present in the original specimen. Rigorous validation Each manufactured lot of this ELISA kit is quality tested for criteria such as sensitivity, specificity, precision, and lot-to-lot consistency. See manual for more information on validation.
Reversibly catalyzes the transfer of phosphate between ATP and various phosphogens (e.g. creatine phosphate). Creatine kinase isoenzymes play a central role in energy transduction in tissues with large, fluctuating energy demands, such as skeletal muscle, heart, brain and spermatozoa.
| Marque | Invitrogen |
|---|---|
| Réf. B-LABO | BL-RE-091747 |
| Réf. fabricant | 18537954 |
| Numéro d’adhésion | P06732,P12277 |
| Plage de dosage | 0.328 to 80 ng/mL |
| Sensibilité du dosage | 6 pg/mL |
| Plage d’étalonnage | 0.3 ng/mL to 80 ng/mL |
| Doser | Solid-phase sandwich ELISA |
| Conjugué | Biotin |
| Type de produit | ELISA Kit |
| Famille de protéines | Kinase & Inhibitors |
| Type d’échantillon | Supernatant |
| Spécificité | This ELISA pair antibody detects human CKMB. Other species not determined. |
| Suffisant pour | 96 assays |
| Espèces cibles | Human |
| À utiliser avec (application) | ELISA, Protein Assays, Protein Biology, Cancer Biology, Immunology, Neuroscience, Neurobiology, Protein Detection, Protein Analysis |
| À utiliser avec (équipement) | Colorimetric Microplate Reader |
| Identification génétique (Entrez) | 1152, 1158 |
| Symbole de gène(s) | B-CK,BCKB,HEL-211,HEL-S-29,CKB,CKMM,M-CK |
| Coefficient de variation inter-dosage | <12% |
| Coefficient de variation intra-dosage | <10% |
| Contenus des kits | Adhesive Plate Covers |
| Marqueur ou colorant | HRP |
| Quantité | 10 x 96 Tests |
| État réglementaire | RUO |
| Disciplines de recherche | Cell Cycle & Proliferation |
| Volume d’échantillon | Plasma, 50 µL; Serum, 50 µL; Supernatant, 100 µL |
| Conditions de stockage | 2°C to 8°C |
| Cible | Human |
| Heure du test | 1 hr. 20 min. |
| Durée totale du dosage | 4 hr. 45 min. |
