Alkaline phosphatase is a homodimeric protein enzyme (86 kDa), containing two zinc atoms crucial to its catalytic function per monomer, and it is optimally active at alkaline pH environments. The enzyme is found in prokaryotes and eukaryotes, with the same general function but in different structural forms suitable to organism's environment. ALP plays a role in liver metabolism and development within the skeleton. In bacteria, ALP acts as a source of inorganic phosphate. In yeast, ALP can be a useful protein for studying membrane protein sorting.
Caractéristiques techniques
| Marque | Invitrogen |
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| Réf. B-LABO | BL-RE-091766 |
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| Réf. fabricant | 18567894 |
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| Numéro d’adhésion | Akp2; Akp-2; Alkaline phosphatase 2; alkaline phosphatase 2, liver; alkaline phosphatase liver/bone/kidney isozyme; alkaline phosphatase precursor (EC 3.1.3.2); alkaline phosphatase tissue non-specific isoform; alkaline phosphatase tissue non-specific isoform/TNS-AP; alkaline phosphatase, biomineralization associated; alkaline phosphatase, liver/bone/kidney; alkaline phosphatase, tissue-nonspecific isozyme; Alpl; APTNAP; AP-TNAP; FLJ40094; FLJ93059; glycerophosphatase; HOPS; liver/bone/kidney isozyme; liver/bone/kidney-type alkaline phosphatase; MGC161443; MGC167935; PHOA; tissue-nonspecific alkaline phosphatase; tissue-nonspecific ALP; Tissue-nonspecific ALP alkaline phosphatase; TNAP; TNSALP; TNS-AP |
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| Plage de dosage | 2 to 500 mIU/mL |
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| Sensibilité du dosage | 2 mIU/mL |
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| Conjugué | Biotin |
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| Type de produit | ELISA |
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| Type d’échantillon | Supernatant |
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| À utiliser avec (équipement) | Colorimetric Microplate Reader |
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| Identification génétique (Entrez) | P05186 |
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| Symbole de gène(s) | ALPL |
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| Coefficient de variation inter-dosage | <12% |
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| Coefficient de variation intra-dosage | <10% |
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| Contenus des kits | Pre-coated 96 well plate, Standard, Assay Diluent concentrate, Biotinylated Detection Antibody, SAV-HRP, Wash Buffer, Chromogen, Stop Solution, Adhesive Plate Covers |
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| Marqueur ou colorant | HRP |
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| Quantité | 10 x 96 Tests |
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| État réglementaire | Research Use Only |
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| Volume d’échantillon | Serum, 50 µL; Plasma, 50 µL; Supernatant, 50 µL |
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| Conditions de stockage | 2°C to 8°C |
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| Cible | ALPL |
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| Heure du test | 1 hr 20 min |
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| Durée totale du dosage | 4 hr 45 min |
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