The Total Antioxidant Capacity (T-AOC) Colorimetric Assay Kit (FRAP method) is a highly reliable and sensitive assay designed for the quantitative measurement of T-AOC in serum, plasma, tissues, cells, cell culture supernatant, saliva, and urine sample types. T-AOC refers to the overall ability of a biological sample to counteract oxidative stress by neutralizing reactive oxygen species (ROS) and other harmful free radicals. It represents the cumulative effect of both enzymatic and non-enzymatic antioxidants present in the sample. The assay quantifies the ability of the sample to reduce ferric ions (Fe3+) to ferrous ions (Fe2+) using the ferric reducing antioxidant power (FRAP) method under acidic conditions. T-AOC can be measured using the absorbance value of the resulting blue solution at 593 nm. Key features • Simple and fast protocol—user-friendly, requiring minimal hands-on time and providing rapid results within a short incubation period • Broad range of kits—across key analytes and targets within the most important metabolic pathways • Consistent—strict quality control criteria to ensure reproducible and reliable results
Caractéristiques techniques
| Marque | Invitrogen |
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| Réf. B-LABO | BL-RE-092409 |
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| Réf. fabricant | 17992031 |
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| Plage de dosage | 0.23–2 mmol Trolox Equiv./L |
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| Sensibilité du dosage | 0.049 mmol/L |
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| Contenu et stockage | Store at 2–8°C.<br><br> |
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| Méthode de détection | Colorimetric |
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| Type d’échantillon | Cell Culture Supernatant, Plasma, Saliva, Serum, Tissue Homogenates, Urine, Whole Cells |
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| À utiliser avec (équipement) | Microplate Reader |
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| Temps d’incubation | 3-5 min. |
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| Coefficient de variation inter-dosage | 8.1 |
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| Coefficient de variation intra-dosage | 3.9 |
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| Volume d’échantillon | 5 μL |
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| Conditions d’expédition | Dry Ice, 2–8°C |
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| Kit cible nommé | Total Antioxidant Capacity (T-AOC) |
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| Durée totale du dosage | 20 min. |
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