Competitive ELISA
The 17-Hydroxyprogesterone Competitive ELISA quantitates 17-Hydroxyprogesterone in serum,plasma,urine,dried fecal extracts or cell culture medium.
The 17-Hydroxyprogesterone Competitive ELISA research-use-only kit uses a specifically generated antibody to measure 17-Hydroxyprogesterone (17HO-P) and its metabolites in reconstituted buffer samples,tissue culture media samples,urine and fecal samples,or in extracted serum and plasma. This kit is not recommended for serum or plasma samples without extraction. A 17HO-P standard is provided to generate a standard curve for the assay and all samples should be read off the standard curve. Standards or diluted samples are pipetted into the provided plate coated with a capture antibody. A 17HO-P-peroxidase conjugate is added to the standards and samples in the wells. The binding reaction is initiated by the addition of a polyclonal antibody to 17HO-P to each well. After a 1-hour incubation the plate is washed and substrate is added. The substrate reacts with the bound 17HO-P-peroxidase conjugate. After a short incubation,the reaction is stopped and the intensity of the generated color is detected in a microtiter plate reader capable of measuring at 450 nm.
Each manufactured lot of this ELISA kit is quality tested for criteria such as sensitivity,specificity,precision,and lot-to-lot consistency. See manual for more information on validation.
17-Hydroxyprogesterone (4-pregen-17-ol-3, 20-dione, 17HO-P, OHPG) is a steroid hormone from the androgen group that is found in mammals, reptiles, birds, and other vertebrates. 17HO-P is identical across all species and so this kit should measure 17HO-P from all sources. In humans, it is primarily synthesized in the adrenal glands, the gonads, testes, and placenta.
| Marque | Invitrogen |
|---|---|
| Réf. B-LABO | BL-RE-092649 |
| Réf. fabricant | 17805117 |
| Plage de dosage | 24,69-6000 pg/ml |
| Sensibilité du dosage | 20,3 pg/ml |
| Conjugué | HRP |
| Type de produit | Kit ELISA |
| Type d’échantillon | Extrait fécal, Sérum, Surnageant, Surnageant, Urine |
| Suffisant pour | 10 x 96 Assays |
| À utiliser avec (application) | ELISA, dosages de protéines, tests d’activité, biologie des protéines, biologie de la reproduction, inflammation, stress oxydatif, métabolisme, signalisation cellulaire, épigénétique, hématologie, dommages à l’ADN, biologie du cancer, immunologie, neurosciences, neurobiologie, détection d’hormones stéroïdes |
| À utiliser avec (équipement) | Lecteur de microplaques colorimétriques |
| Coefficient de variation inter-dosage | 8% |
| Coefficient de variation intra-dosage | 6,6% |
| Contenus des kits | Plaque à 96 puits pré-enduite, Étalon, Concentrat de diluant de dosage, Anticorps de détection conjugué au HRP, Tampon de lavage, Chromogène, Solution d’arrêt, Couvercles de plaques adhésives |
| Marqueur ou colorant | HRP |
| État réglementaire | RUO |
| Disciplines de recherche | Biologie de la reproduction |
| Volume d’échantillon | Extrait fécal, 50 μl ; plasma, 50 μl ; sérum, 50 μl ; surnageant, 50 μl ; urine, 25 μl |
| Conditions de stockage | -20°C |
| Cible | Produits chimiques |
| Heure du test | 1 h 20 min |
| Durée totale du dosage | 1 h 30 min |
