"/> Invitrogen™ Kit ELISA HGF de souris | B-LABO France

Invitrogen™ Kit ELISA HGF de souris

Réf. : BL-RE-092670Réf. fabricant : 18597814 10 x 96 Tests
4 815,00 € HT

Sandwich ELISA Kit

Numéro d’adhésion
Q08048
Plage de dosage
0.614 to 60 ng/mL
Sensibilité du dosage
400 pg/mL
Plage d’étalonnage
400 pg/mL to 60 ng/mL
Description
HGF ELISA Kit, 96 Assays, 1 Kit, Mouse Species, Chemokines and Receptors Protein Family, Colorimetric Detection Method, Enzyme-Linked Immunosorbent Assay Designed for the Quantitation and Detection of HGF in Samples
Marque
Invitrogen

The Mouse Hepatocyte Growth Factor(Ms HGF) ELISA quantitates Ms HGF in mouse serum, plasma, or cell culture medium. The assay will exclusively recognize both natural and recombinant Ms HGF. Principle of the method The Mouse HGF solid-phase sandwich ELISA (enzyme-linked immunosorbent assay) is designed to measure the amount of the target bound between a matched antibody pair. A target-specific antibody has been pre-coated in the wells of the supplied microplate. Samples, standards, or controls are then added into these wells and bind to the immobilized (capture) antibody. The sandwich is formed by the addition of the second (detector) antibody, a substrate solution is added that reacts with the enzyme-antibody-target complex to produce measurable signal. The intensity of this signal is directly proportional to the concentration of target present in the original specimen. Rigorous validation Each manufactured lot of this ELISA kit is quality tested for criteria such as sensitivity, specificity, precision, and lot-to-lot consistency. See manual for more information on validation.

Hepatocyte growth factor (HGF) is a member of the plasminogen subfamily of S1 peptidases. The inactive polypeptide formed upon translation undergoes cleavage to produce a 69 kDa alpha-chain and 34 kDa beta-chain. A disulphide bond between the two chains makes active HGF. HGF is secreted by mesenchymal cells and stimulates mitogenesis, cell motility, and matrix invasion. It is involved in cellular growth and morphogenesis in a wide variety of tissue types. In humans, HGF is present on chromosome 7.

Caractéristiques techniques

MarqueInvitrogen
Réf. B-LABOBL-RE-092670
Réf. fabricant18597814
Numéro d’adhésionQ08048
Plage de dosage0.614 to 60 ng/mL
Sensibilité du dosage400 pg/mL
Plage d’étalonnage400 pg/mL to 60 ng/mL
Substances à analyser détectablesHGF
DoserSolid-phase sandwich ELISA
ConjuguéBiotin
Méthode de détectionColorimetric
Format du kit d’immunoessaiSandwich ELISA Kit
Type de produitELISA Kit
Famille de protéinesChemokine & Receptors
Type d’échantillonSupernatant
SpécificitéThis ELISA kit shows no cross-reactivity with the following cytokines tested: Mouse CD30, L CD30, T CD40, CRG-2, CTACK, CXCL16, Eotaxin , Eotaxin-2, Fas Ligand, Fractalkine, GCSF, GM-CFS, IFN- gamma, IGFBP-3, IGFBP-5, IGFBP-6, IL-1 alpha, IL-1 beta, IL-2, IL-3, IL-3 Rb, IL-4, IL-5, IL-9, IL-10, IL-12 p40/p70, IL-12 p70, IL-13, IL-17, KC, Leptin R, LEPTIN(OB), LIX, L-Selectin, Lymphotactin, MCP-1, MCP- 5, M-CSF, MIG, MIP-1 alpha, MIP-1 gamma, MIP-2, MIP-3 beta, MIP-3 alpha, PF-4, PSelectin, RANTES, SCF, SDF-1 alpha, TARC, TCA-3, TECK, TIMP-1, TNF-alpha, TNF RI, TNF RII, TPO, VCAM-1, VEGF.
Suffisant pour96 Assays
Espèces ciblesMouse
À utiliser avec (application)ELISA, Protein Assays, Protein Biology, Cancer Biology, Immunology, Neuroscience, Neurobiology, Protein Detection, Protein Analysis
À utiliser avec (équipement)Colorimetric Microplate Reader
Identification génétique (Entrez)15234
Symbole de gène(s)C230052L06Rik,HGF/SF,NK1,NK2,SF,SF/HGF
Espèces hôtesMouse
Coefficient de variation inter-dosage<12%
Coefficient de variation intra-dosage<10%
Contenus des kitsSAV-HRP
Marqueur ou colorantHRP
Quantité10 x 96 Tests
État réglementaireRUO
Disciplines de rechercheApoptosis
Volume d’échantillonPlasma, 50 µL; Serum, 50 µL; Supernatant, 100 µL
Conditions de stockage2°C to 8°C
SynonymeC230052L06Rik, HGF/SF, NK1, NK2, SF, SF/HGF
CibleMouse
Heure du test1 hr. 20 min.
Durée totale du dosage4 hr. 45 min.
Une question, une configuration particulière ?
Contactez-nous pour un devis.
Demander un devis 03 65 67 15 79

Produits similaires

Invitrogen&trade;&nbsp;Kit ELISA Calcitonin de singe

Invitrogen™ Kit ELISA Calcitonin de singe

638,00 € HT
Invitrogen&trade;&nbsp;Kit ELISA Transthyretin de singe

Invitrogen™ Kit ELISA Transthyretin de singe

638,00 € HT
Invitrogen&trade;&nbsp;Kit ELISA P1NP humain

Invitrogen™ Kit ELISA P1NP humain

638,00 € HT
Invitrogen&trade;&nbsp;Kit ELISA NEFL de rat

Invitrogen™ Kit ELISA NEFL de rat

638,00 € HT