Sandwich ELISA Kit
The Mouse PLGF-2 (PGF) ELISA quantitates Ms PLGF-2 in mouse serum, plasma, or cell culture medium. The assay will exclusively recognize both natural and recombinant Ms PLGF-2. Principle of the method The Mouse PLGF-2 solid-phase sandwich ELISA (enzyme-linked immunosorbent assay) is designed to measure the amount of the target bound between a matched antibody pair. A target-specific antibody has been pre-coated in the wells of the supplied microplate. Samples, standards, or controls are then added into these wells and bind to the immobilized (capture) antibody. The sandwich is formed by the addition of the second (detector) antibody, a substrate solution is added that reacts with the enzyme-antibody-target complex to produce measurable signal. The intensity of this signal is directly proportional to the concentration of target present in the original specimen. Rigorous validation Each manufactured lot of this ELISA kit is quality tested for criteria such as sensitivity, specificity, precision, and lot-to-lot consistency. See manual for more information on validation.
Placental growth factor (PlGF) is a member of the vascular endothelial growth factor (VEGF) family. PlGF is a pleiotropic factor affects different cell types and regulates various biological responses. The activity of vascular endothelial growth factor (VEGF), which is the most potent of all angiogenic mediators, is partly modulated by PlGF. It plays a major role in vasculogenesis and angiogenesis in human placenta.
| Marque | Invitrogen |
|---|---|
| Réf. B-LABO | BL-RE-092688 |
| Réf. fabricant | 18587924 |
| Numéro d’adhésion | P49764 |
| Plage de dosage | 1.64 to 400 pg/mL |
| Sensibilité du dosage | 1 pg/mL |
| Plage d’étalonnage | 1 pg/mL to 400 pg/mL |
| Doser | Solid-phase sandwich ELISA |
| Conjugué | Biotin |
| Type de produit | ELISA Kit |
| Famille de protéines | Growth Factors & Receptors |
| Type d’échantillon | Plasma |
| Spécificité | The sandwich ELISA antibody pair detects Mouse PLGF-2. |
| Suffisant pour | 96 assays |
| Espèces cibles | Mouse |
| À utiliser avec (application) | ELISA, Protein Assays, Protein Biology, Cancer Biology, Immunology, Neuroscience, Neurobiology, Protein Detection, Protein Analysis |
| À utiliser avec (équipement) | Colorimetric Microplate Reader |
| Identification génétique (Entrez) | 18654 |
| Symbole de gène(s) | Pgf |
| Coefficient de variation inter-dosage | <12% |
| Coefficient de variation intra-dosage | <10% |
| Contenus des kits | Standard |
| Marqueur ou colorant | HRP |
| Quantité | 10 x 96 Tests |
| État réglementaire | RUO |
| Disciplines de recherche | Development |
| Volume d’échantillon | Plasma, 50 µL; Serum, 50 µL; Supernatant, 100 µL |
| Conditions de stockage | 2°C to 8°C |
| Synonyme | AI854365, PIGF, Plgf |
| Cible | Mouse |
| Heure du test | 1 hr. 20 min. |
| Durée totale du dosage | 4 hr. 45 min. |
