Sandwich ELISA Kit
The Rat TCK-1 (PPBP) ELISA quantitates Rt TCK-1 in rat serum, plasma, or cell culture medium. The assay will exclusively recognize both natural and recombinant Rt TCK-1. Principle of the method The Rat TCK-1 solid-phase sandwich ELISA (enzyme-linked immunosorbent assay) is designed to measure the amount of the target bound between a matched antibody pair. A target-specific antibody has been pre-coated in the wells of the supplied microplate. Samples, standards, or controls are then added into these wells and bind to the immobilized (capture) antibody. The sandwich is formed by the addition of the second (detector) antibody, a substrate solution is added that reacts with the enzyme-antibody-target complex to produce measurable signal. The intensity of this signal is directly proportional to the concentration of target present in the original specimen. Rigorous validation Each manufactured lot of this ELISA kit is quality tested for criteria such as sensitivity, specificity, precision, and lot-to-lot consistency. See manual for more information on validation.
Members of the a-chemokine subfamily of inducible, secreted, pro-inflammatory cytokines contain a similar motif, in which the first two cysteine residues are separated by a single residue (Cys-X-Cys), and are also chemotactic for neutrophils. The platelet basic protein (PBP), a member of the a-chemokine family, resides in the a-granules of platelets and is released upon their activation. Proteolytic cleavage of the amino terminus of PBP leads to the generation of several peptides, which include mature PBP, connective tissue-activating peptide III (CTAP III, also designated low affinity platelet factor IV (LA-PF4)), b-thromboglobulin (b-TG), and neutrophil-activating peptide 2 (NAP-2). PBP and its N-truncated derivatives mediate inflammation and wound healing. Specifically, NAP-2 activates chemotaxis and degranulation in neutrophils during inflammation. The gene encoding human PBP maps to chromosome 4q12-q13.
| Marque | Invitrogen |
|---|---|
| Réf. B-LABO | BL-RE-092990 |
| Réf. fabricant | 18577834 |
| Numéro d’adhésion | NP_714943.1 |
| Plage de dosage | 68.59 pg/mL to 50,000 pg/mL |
| Sensibilité du dosage | 80 pg/mL |
| Plage d’étalonnage | 80 pg/mL to 50,000 pg/mL |
| Doser | Solid-phase sandwich ELISA |
| Conjugué | Biotin |
| Type de produit | ELISA Kit |
| Famille de protéines | Chemokine & Receptors |
| Type d’échantillon | Serum |
| Spécificité | This ELISA kit shows no cross-reactivity with the following cytokines tested: rat CINC-2, CINC-3, CNTF, Fractalkine, IL-1 alpha, IL-1 beta, IL-4, IL-6, IL-10, GM-CSF, IFN-gamma, Leptin, Lix, MCP-1, MIP-3 alpha, beta- NGF, TIMP-1, TNF-alpha. |
| Suffisant pour | 96 assays |
| À utiliser avec (application) | ELISA, Protein Assays, Protein Biology, Cancer Biology, Immunology, Neuroscience, Neurobiology, Protein Detection, Protein Analysis |
| À utiliser avec (équipement) | Colorimetric Microplate Reader |
| Identification génétique (Entrez) | 246358 |
| Symbole de gène(s) | Ppbp |
| Coefficient de variation inter-dosage | <12% |
| Coefficient de variation intra-dosage | <10% |
| Contenus des kits | Chromogen |
| Marqueur ou colorant | HRP |
| Quantité | 10 x 96 Tests |
| État réglementaire | RUO |
| Disciplines de recherche | Immunology |
| Volume d’échantillon | Plasma, 1 µL; Serum, 1 µL; Supernatant, 100 µL |
| Conditions de stockage | 2°C to 8°C |
| Synonyme | Cxcl7, Nap-2 |
| Cible | Rat |
| Heure du test | 1 hr. 20 min. |
| Durée totale du dosage | 4 hr. 45 min. |
