Sandwich ELISA Kit
The Mouse Galectin-1 (LGALS1) ELISA quantitates Ms Galectin-1 in mouse serum, plasma, or cell culture medium. The assay will exclusively recognize both natural and recombinant Ms Galectin-1. Principle of the method The Mouse Galectin-1 solid-phase sandwich ELISA (enzyme-linked immunosorbent assay) is designed to measure the amount of the target bound between a matched antibody pair. A target-specific antibody has been pre-coated in the wells of the supplied microplate. Samples, standards, or controls are then added into these wells and bind to the immobilized (capture) antibody. The sandwich is formed by the addition of the second (detector) antibody, a substrate solution is added that reacts with the enzyme-antibody-target complex to produce measurable signal. The intensity of this signal is directly proportional to the concentration of target present in the original specimen. Rigorous validation Each manufactured lot of this ELISA kit is quality tested for criteria such as sensitivity, specificity, precision, and lot-to-lot consistency. See manual for more information on validation.
Galectin-1 belongs to a large family of carbohydrate-binding proteins called lectins. Galectin-1can be either monomeric or homodimeric and is found in a wide variety of cells and tissue types. Galectin-1 can control cell growth, proliferation, induce apoptosis of activated T cells while it can also modulate cytokine secretion or inhibit pro-inflammatory cytokine production. Galectin-1 plays an important role in acute and chronic inflammation.
| Marque | Invitrogen |
|---|---|
| Réf. B-LABO | BL-RE-093326 |
| Réf. fabricant | 18547784 |
| Numéro d’adhésion | P16045 |
| Plage de dosage | 16.38 to 4000 pg/mL |
| Sensibilité du dosage | 12 pg/mL |
| Plage d’étalonnage | 2 to 10 fold |
| Doser | Solid-phase sandwich ELISA |
| Conjugué | Biotin |
| Type de produit | ELISA Kit |
| Famille de protéines | Other Proteins |
| Type d’échantillon | Plasma |
| Spécificité | The antibody pair provided in this kit recognizes mouse Galectin-1. |
| Suffisant pour | 96 assays |
| Espèces cibles | Mouse |
| À utiliser avec (application) | ELISA, Protein Assays, Protein Biology, Cancer Biology, Immunology, Neuroscience, Neurobiology, Protein Detection, Protein Analysis |
| À utiliser avec (équipement) | Colorimetric Microplate Reader |
| Identification génétique (Entrez) | 16852 |
| Symbole de gène(s) | AA410090,Gal-1,Galbp,L-14.5,L14,Lect14,galectin-1 |
| Coefficient de variation inter-dosage | <12% |
| Coefficient de variation intra-dosage | <10% |
| Contenus des kits | Assay Diluent concentrate |
| Marqueur ou colorant | HRP |
| Quantité | 10 x 96 Tests |
| État réglementaire | RUO |
| Disciplines de recherche | Apoptosis |
| Volume d’échantillon | Plasma, 1 µL; Serum, 1 µL; Supernatant, 100 µL |
| Conditions de stockage | 2°C to 8°C |
| Synonyme | AA410090, Gal-1, Galbp, L-14.5, L14, Lect14, galectin-1 |
| Cible | Mouse |
| Heure du test | 1 hr. 20 min. |
| Durée totale du dosage | 4 hr. 45 min. |
