Interleukin 10 (IL-10, CSIF) is an anti-inflammatory cytokine mainly produced by macrophages and Th2 cells. IL-10 is an 18.6 kDa protein of 160 amino acid residues that shares over 80% sequence homology with the Epstein-Barr Virus protein (BCRFI). The reported biological activities of IL-10, which maybe interrelated, include inhibition of macrophage-mediated cytokine synthesis, suppression of the delayed-type hyper-sensitivity response, and stimulation of the Th2 cell response which results in elevated antibody production. IL-10 functions by inhibiting pro-inflammatory cytokines made by macrophages and regulatory T cells including IFN-gamma, TNF-alpha, IL-2, and IL-3, IL-4, and GM-CSF. IL-10 is also known to suppress antigen presentation on antigen presenting cells, enhances B cell survival, proliferation, and antibody production. IL-10 can block NF-kappa B activity, and is involved in the regulation of the JAK-STAT signaling pathway. While human IL-10 can act on murine cells, murine IL-10 cannot act on human cells. Binds to a retinoid X receptor (RXR) responsive element from the cellular retinol-binding protein II promoter (CRBPII-RXRE). IL-10 inhibits the 9-cis-retinoic acid-dependent RXR alpha transcription activation of the retinoic acid responsive element. Knockout studies in mice suggest the function of this cytokine as an essential immunoregulator in the intestinal tract.
Caractéristiques techniques
| Marque | Invitrogen |
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| Réf. B-LABO | BL-RE-093396 |
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| Réf. fabricant | 18537894 |
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| Numéro d’adhésion | CSIF; cytokine; cytokine synthesis inhibitory factor; GVHDS; H-IL-10; il 10; IL10; IL-10; IL-10 precursor; IL10A; IL10X; ILN; Interleukin; interleukin 10; Interleukin10; Interleukin-10; MGC126450; MGC126451; RP11-262N9.1; T-cell growth inhibitory factor; TGIF |
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| Plage de dosage | 10 to 6,000 pg/mL |
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| Sensibilité du dosage | 10 pg/mL |
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| Conjugué | Biotin |
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| Type de produit | ELISA |
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| Type d’échantillon | Plasma |
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| À utiliser avec (équipement) | Colorimetric Microplate Reader |
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| Identification génétique (Entrez) | P29456 |
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| Symbole de gène(s) | IL10 |
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| Coefficient de variation inter-dosage | <12% |
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| Coefficient de variation intra-dosage | <10% |
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| Contenus des kits | Pre-coated 96 well plate, Standard, Assay Diluent concentrate, Biotinylated Detection Antibody, SAV-HRP, Wash Buffer, Chromogen, Stop Solution, Adhesive Plate Covers |
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| Marqueur ou colorant | HRP |
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| Quantité | 10 x 96 Tests |
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| État réglementaire | Research Use Only |
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| Volume d’échantillon | Supernatant, 50 µL; Plasma, 50 µL; Serum, 50 µL |
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| Conditions de stockage | 2°C to 8°C |
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| Cible | IL-10 |
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| Heure du test | 1 hr 20 min |
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| Durée totale du dosage | 4 hr 45 min |
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