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Invitrogen™ Kit ELISA Complement Factor D/Adipsin humain

Réf. : BL-RE-093401Réf. fabricant : 18547954 10 x 96 Tests
4 815,00 € HT

Sandwich ELISA Kit

Numéro d’adhésion
P00746
Plage de dosage
8.23 to 6000 pg/mL
Sensibilité du dosage
4 pg/mL
Plage d’étalonnage
4 pg/mL to 6000 pg/mL
Description
CFD (Adipsin) ELISA Kit, 96 Assays, 1 Kit, Human Species, Other Proteins Protein Family, Other Research Discipline, Enzyme-Linked Immunosorbent Assay Designed for the Quantitation and Detection of Cfd (Adipsin) in Samples, Using a Microplate Reader
Marque
Invitrogen

The Human Complement Factor D (Adipsin/CFD) ELISA quantitates Hu Complement Factor D in human serum, plasma, or cell culture medium. The assay will exclusively recognize both natural and recombinant Hu Complement Factor D. Principle of the method The Human Complement Factor D solid-phase sandwich ELISA (enzyme-linked immunosorbent assay) is designed to measure the amount of the target bound between a matched antibody pair. A target-specific antibody has been pre-coated in the wells of the supplied microplate. Samples, standards, or controls are then added into these wells and bind to the immobilized (capture) antibody. The sandwich is formed by the addition of the second (detector) antibody, a substrate solution is added that reacts with the enzyme-antibody-target complex to produce measurable signal. The intensity of this signal is directly proportional to the concentration of target present in the original specimen. Rigorous validation Each manufactured lot of this ELISA kit is quality tested for criteria such as sensitivity, specificity, precision, and lot-to-lot consistency. See manual for more information on validation.

Factor D is a serine protease of the alternative pathway of complement activation. The protein is a single chain polypeptide with a molecular mass of 25 kDa. Factor D cleaves factor B bound to C3b, generating the alternative pathway C3 convertase C3bBb and releasing the Ba fragment.

Caractéristiques techniques

MarqueInvitrogen
Réf. B-LABOBL-RE-093401
Réf. fabricant18547954
Numéro d’adhésionP00746
Plage de dosage8.23 to 6000 pg/mL
Sensibilité du dosage4 pg/mL
Plage d’étalonnage4 pg/mL to 6000 pg/mL
Substances à analyser détectablesCFD (Adipsin)
DoserSolid-phase sandwich ELISA
ConjuguéBiotin
Méthode de détectionColorimetric
Type de produitELISA Kit
Famille de protéinesOther Proteins
Type d’échantillonSerum
SpécificitéThis ELISA kit shows no cross-reactivity with the following cytokines tested: human Angiogenin, BDNF, BLC, ENA-78, FGF- 4, IL-1 alpha, IL-1 beta, IL-2, IL-3, IL-4, IL-5, IL-6, IL-7, IL-8, IL-9, IL-11, IL-12 p70, IL-12 p40, IL-13, IL-15, IL-309, IP-10, G-CSF, GM-CSF, IFN-gamma, Leptin (OB), MCP-1, MCP-2, MCP-3, MDC, MIP-1 alpha, MIP-1 beta, MIP-1 delta, PARC, PDGF, RANTES, SCF, TARC, TGF-beta, TIMP-1, TIMP-2, TNF-alpha, TNF-beta, TPO, VEGF
Type de substratTMB
Suffisant pour96 Assays
Espèces ciblesHuman
À utiliser avec (application)ELISA, Protein Assays, Protein Biology, Cancer Biology, Immunology, Neuroscience, Neurobiology, Protein Detection, Protein Analysis
À utiliser avec (équipement)Colorimetric Microplate Reader
Identification génétique (Entrez)1675
Symbole de gène(s)ADIPSIN,ADN,DF,PFD
Espèces hôtesHuman
Coefficient de variation inter-dosage<12%
Coefficient de variation intra-dosage<10%
Contenus des kitsStop Solution
Marqueur ou colorantHRP
Quantité10 x 96 Tests
État réglementaireRUO
Disciplines de rechercheOther Research
Volume d’échantillonPlasma, 1 µL; Serum, 1 µL; Supernatant, 100 µL
Conditions de stockage2°C to 8°C
SynonymeADIPSIN, ADN, DF, PFD
CibleHuman
Heure du test1 hr. 20 min.
Durée totale du dosage4 hr. 45 min.
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