The Lactate Dehydrogenase (LDH) Colorimetric Assay Kit is a highly reliable and sensitive assay designed for the quantitative measurement of LDH in serum, plasma, tissue, and cell culture samples. LDH is an enzyme that catalyzes the conversion of lactate to pyruvate, an essential step in cellular energy metabolism. Elevated LDH levels are associated with tissue damage, inflammation, and certain diseases, making it a useful biomarker in clinical and research settings. In this assay, LDH catalyzes lactic acid to produce pyruvate. Pyruvate reacts with 2, 4-dinitrophenylhydrazine to form pyruvate dinitrophenylhydrazone, which is red-brown in alkaline solution. The color intensity is proportional to pyruvate concentration, where the activity of LDH can be calculated by measuring OD value. Key features • Simple and fast protocol—user-friendly, requiring minimal hands-on time and providing rapid results within a short incubation period • Broad range of kits—across key analytes and targets within the most important metabolic pathways • Consistent—strict quality control criteria to ensure reproducible and reliable results
Caractéristiques techniques
| Marque | Invitrogen |
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| Réf. B-LABO | BL-RE-093739 |
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| Réf. fabricant | 17982071 |
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| Plage de dosage | 6–1000 U/L |
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| Sensibilité du dosage | 6 U/L |
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| Contenu et stockage | Store at 2–8°C.<br><br> |
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| Méthode de détection | Colorimetric |
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| Type d’échantillon | Hydrothorax, Plasma, Serum, Tissue Homogenates, Whole Cells |
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| À utiliser avec (équipement) | Microplate Reader |
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| Temps d’incubation | 35 min. |
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| Coefficient de variation inter-dosage | 2.4 |
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| Coefficient de variation intra-dosage | 1.8 |
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| Volume d’échantillon | 40 μL |
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| Conditions d’expédition | Dry Ice, 2–8°C |
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| Kit cible nommé | Lactate Dehydrogenase (LDH) |
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| Durée totale du dosage | 45 min. |
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