The Mouse SDF-1 alpha/CXCL12 ELISA quantitates Ms SDF-1α in mouse serum, plasma, or cell culture medium. The assay will exclusively recognize both natural and recombinant Ms SDF-1α. Principle of the method The Mouse SDF-1α solid-phase sandwich ELISA (enzyme-linked immunosorbent assay) is designed to measure the amount of the target bound between a matched antibody pair. A target-specific antibody has been pre-coated in the wells of the supplied microplate. Samples, standards, or controls are then added into these wells and bind to the immobilized (capture) antibody. The sandwich is formed by the addition of the second (detector) antibody, a substrate solution is added that reacts with the enzyme-antibody-target complex to produce measurable signal. The intensity of this signal is directly proportional to the concentration of target present in the original specimen. Rigorous validation Each manufactured lot of this ELISA kit is quality tested for criteria such as sensitivity, specificity, precision, and lot-to-lot consistency. See manual for more information on validation.
CXCL12 is a stromal cell-derived alpha chemokine member of the intercrine family. This gene product and its receptor CXCR4 can activate lymphocytes and have been implicated in the metastasis of some cancers such as breast cancer. Mutations in this gene are associated with resistance to human immunodeficiency virus type 1 infections. Multiple transcript variants encoding different isoforms have been found for this gene.
Caractéristiques techniques
| Marque | Invitrogen |
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| Réf. B-LABO | BL-RE-093759 |
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| Réf. fabricant | 18507924 |
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| Numéro d’adhésion | P40224 |
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| Plage de dosage | 9.38 to 600 pg/mL |
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| Sensibilité du dosage | 3 pg/mL |
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| Plage d’étalonnage | 3 pg/mL to 600 pg/mL |
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| Substances à analyser détectables | SDF-1 alpha CXCL12, mouse SDF-1 |
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| Doser | Solid-phase sandwich ELISA |
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| Conjugué | Biotin |
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| Méthode de détection | Colorimetric |
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| Type de produit | ELISA Kit |
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| Famille de protéines | Chemokine & Receptors |
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| Type d’échantillon | Supernatant |
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| Spécificité | This ELISA kit shows no cross-reactivity with any of the cytokines tested: Mouse CD30, L CD30, T CD40, CRG-2, CTACK, CXCL16, Eotaxin, Eotaxin-2, Fas Ligand, Fractalkine, GCSF, GM-CFS, IFN-gamma, IGFBP-3, IGFBP-5, IGFBP-6, IL-1 alpha, IL-1 beta, IL-2, IL-3 |
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| Suffisant pour | 96 Assays |
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| Espèces cibles | Mouse |
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| À utiliser avec (application) | ELISA, Protein Assays, Protein Biology, Cancer Biology, Immunology, Neuroscience, Neurobiology, Protein Detection, Protein Analysis |
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| À utiliser avec (équipement) | Colorimetric Microplate Reader |
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| Identification génétique (Entrez) | 20315 |
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| Symbole de gène(s) | Pbsf,Scyb12,Sdf1,Tlsf,Tpar1 |
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| Espèces hôtes | Mouse |
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| Coefficient de variation inter-dosage | <12% |
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| Coefficient de variation intra-dosage | <10% |
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| Contenus des kits | SAV-HRP |
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| Marqueur ou colorant | HRP |
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| Quantité | 10 x 96 Tests |
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| État réglementaire | RUO |
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| Disciplines de recherche | Immunology |
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| Volume d’échantillon | Plasma, 50 µL; Serum, 50 µL; Supernatant, 100 µL |
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| Conditions de stockage | 2°C to 8°C |
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| Synonyme | Pbsf, Scyb12, Sdf1, Tlsf, Tpar1 |
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| Cible | Mouse |
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| Heure du test | 1 hr. 20 min. |
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| Durée totale du dosage | 4 hr. 45 min. |
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