Pour la détection quantitative de sPECAM-1 humaine.
The Human Soluble CD31/Platelet Endothelial Cell Adhesion Molecule(Hu sCD31/PECAM) ELISA quantitates Hu sCD31/PECAM in human serum, plasma, cell culture supernatants, urine, or other body fluids. The assay will exclusively recognize both natural and recombinant Hu sCD31/PECAM. Principle of the method The Human sCD31/PECAM solid-phase sandwich ELISA (enzyme-linked immunosorbent assay) is designed to measure the amount of the target bound between a matched antibody pair. A target-specific antibody has been pre-coated in the wells of the supplied microplate. Samples or controls are then added into these wells and bind to the immobilized (capture) antibody. The sandwich is formed by the binding of the second (detector) antibody to the target on a different epitope from the capture antibody. An antibody conjugated with enzyme binds the formed sandwich. After incubation and washing steps to rid the microplate of unbound substances, a substrate solution is added that reacts with the enzyme-antibody-target complex to produce measurable signal. The intensity of this signal is directly proportional to the concentration of target present in the original specimen. Rigorous validation Each manufactured lot of this ELISA kit is quality tested for criteria such as sensitivity, specificity, precision, and lot-to-lot consistency. See manual for more information on validation.
Les kits Platinum subissent des processus rigoureux de développement, de fabrication et de validation de la qualité, garantissant des performances de produit optimales et une cohérence optimale entre les lots.
| Marque | Invitrogen |
|---|---|
| Réf. B-LABO | BL-RE-096794 |
| Réf. fabricant | 15501777 |
| Numéro d’adhésion | P16284 |
| Plage de dosage | 0,47-30,0 ng/ml |
| Sensibilité du dosage | 0,06 ng |
| Conjugué | HRP |
| Type de produit | Kit ELISA |
| Type d’échantillon | Sérum, Surnageant, Supernatant, Urine |
| Espèces cibles | Humaine |
| À utiliser avec (application) | ELISA |
| À utiliser avec (équipement) | Lecteur de microplaques colorimétriques |
| Identification génétique (Entrez) | 5175 |
| Symbole de gène(s) | CD31,CD31/EndoCAM,GPIIA′,PECA1,PECAM-1,endoCAM |
| Coefficient de variation inter-dosage | 7.4% |
| Coefficient de variation intra-dosage | 1.7% |
| Contenus des kits | Plaque à 96 puits pré-enduite, Étalon, Diluant d’échantillon, Concentrat de tampon de dosage, Anticorps de détection conjugué au HRP, Tampon de lavage, Chromogène, Solution d’arrêt, isotypiques, Couvercles de plaques adhésives |
| Marqueur ou colorant | HRP |
| Quantité | 10 x 96 essais |
| État réglementaire | RUO |
| Volume d’échantillon | Plasma, 10 μl ; Sérum, 10 μl ; Surnageant, 10 μl ; Urine, 10 μl |
| Conditions de stockage | 2°C à 8°C |
| Synonyme | Protéine de surface des leucocytes, CD31, EndoCAM, HEC7 |
| Cible | Humaine |
| Heure du test | 1 h 20 min |
| Durée totale du dosage | 3 h 10 min |
