For detection and measurement of human interleukin 12 (p70) subunit
Description
B Cells; Dendritic Cells; Granulocytes and Subsets; Macrophages; Monocytes
À utiliser avec (application)
ELISA
Quantité
2 Plates
Marque
STEMCELL Technologies
Caractéristiques principales
The Human Interleukin 12 (IL-12) p70 ELISA Kit is designed for the quantitative detection and measurement of human IL-12 (p70) in biological fluids such as serum, plasma, and cell culture supernatants
IL-12 (p70) is a heterodimeric cytokine composed of p35 and p40 subunits; the p40 subunit is shared with IL-23
IL-12 is produced by monocytes, macrophages, dendritic cells, neutrophils, and B cells
The assay is based on the sandwich ELISA method, in which samples are added to ELISA strip plates pre-coated with capture antibodies specific for the cytokine
The captured cytokine is detected by addition of a biotinylated detection antibody, followed by streptavidin-horseradish peroxidase, which binds the biotinylated antibody
Addition of the chromogenic enzyme substrate 3,3',5,5' tetramethylbenzidine (TMB) results in a colored product with an intensity directly proportional to the concentration of cytokine in the sample
Caractéristiques techniques
Marque
STEMCELL Technologies
Réf. B-LABO
BL-RE-097288
Réf. fabricant
17168021
À utiliser avec (application)
ELISA
Quantité
2 Plates
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