For detection and measurement of human interleukin 4
Description
Granulocytes and Subsets; NK Cells; T Cells, Other Subsets
À utiliser avec (application)
ELISA
Quantité
2 Plates
Marque
STEMCELL Technologies
Caractéristiques principales
The Human Interleukin 4 (IL-4) ELISA Kit is designed for the quantitative detection and measurement of human IL-4 in biological fluids such as serum, plasma, and cell culture supernatants
IL-4 is produced by Th2 cells, NK cells, γ/δ T cells, eosinophils, mast cells, and activated basophils
IL-4 induces Ig isotype switching in B cells, regulates the differentiation of naïve T cells into Th2 cells
The assay is based on the sandwich ELISA method, in which samples are added to ELISA strip plates pre-coated with capture antibodies specific for the cytokine
The captured cytokine is detected by addition of a biotinylated detection antibody, followed by streptavidin-horseradish peroxidase, which binds the biotinylated antibody
Addition of the chromogenic enzyme substrate 3,3',5,5' tetramethylbenzidine (TMB) results in a colored product with an intensity directly proportional to the concentration of cytokine in the sample
Caractéristiques techniques
Marque
STEMCELL Technologies
Réf. B-LABO
BL-RE-097302
Réf. fabricant
17168011
À utiliser avec (application)
ELISA
Quantité
2 Plates
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