"/> Invitrogen™ Kit ELISA IL-1 beta de souriss | B-LABO France

Invitrogen™ Kit ELISA IL-1 beta de souriss

Réf. : BL-RE-097309Réf. fabricant : 11894614 1 Plate Kit
564,90 € HT

Measure levels of mouse interleukin-1 beta (IL-1b) in mouse serum, plasma (EDTA, heparin, sodium citrate) and culture supernatant samples using 96-well plates.

Plage de dosage
31.2 to 2000pg/mL
Sensibilité du dosage
<3pg/mL
Description
Mouse IL-1 beta ELISA Kit
Format du kit d’immunoessai
Colorimetric ELISA (TMB substrate) with Plates
Type d’échantillon
Cell Culture Supernatant, Plasma (EDTA, Citrate, Heparin), Serum
Marque
Invitrogen

Assay IL-1β (mouse interleukin-1 beta) levels in mouse serum, plasma (EDTA, heparin, sodium citrate) and culture supernatant samples with our Thermo Scientific Mouse IL-1 beta ELISA Kits.

These mouse IL-1 beta ELISA kits use pre-titered, matched pairs of coating and detection antibodies to achieve sensitive and accurate measurement of interleukin-1 beta (IL-1β) in samples at 50μL per well. Also included are calibrated recombinant protein standard, streptavidin-HRP, TMB substrate solution and stop solution. The complete kits contain pre-coated 96-well strip plate(s) and lot-optimized reagents. All components undergo quality control testing to minimize lot-to-lot variability.

Includes:Complete kits contain pre-coated antibody-plates, detection antibodies, buffers, diluents, standards, and substrates.

Caractéristiques principales

  • Target: Natural and recombinant mouse interleukin-1 beta (IL-1β)
  • Format: Colorimetric ELISA (TMB substrate) in clear 96-well strip plate
  • Measurement: Absorbance at 450nm minus absorbance at 550nm
  • Assay Range: 31.2 to 2000pg/mL
  • Sensitivity: < 3pg/mL
  • Precision: Intra-assay CV ≤ 10%; inter-assay CV ≤ 10%
  • Sample Types: Cell culture supernatant, serum, plasma (EDTA, sodium citrate and heparin)
  • Total Assay Time: 4 hours
  • Sample Size: 50μL
  • Specificity: No significant cross-reaction with mouse IL-1α, IL-10, IL-2, IL-4, IL-6, TNFα, GM-CSF; human IL-1β, IL-8, IL-1α; or bovine IL-1β. This ELISA does cross-react with rat IL-1β (12%)

Caractéristiques techniques

MarqueInvitrogen
Réf. B-LABOBL-RE-097309
Réf. fabricant11894614
Plage de dosage31.2 to 2000pg/mL
Sensibilité du dosage<3pg/mL
Format du kit d’immunoessaiColorimetric ELISA (TMB substrate) with Plates
Type d’échantillonCell Culture Supernatant, Plasma (EDTA, Citrate, Heparin), Serum
SpécificitéNo significant cross-reaction with mouse IL-1a, IL-10, IL-2, IL-4, IL-6, TNFa, GM-CSF; human IL-1ß, IL-8, IL-1a; or bovine IL-1ß. This ELISA does cross-react with rat IL-1ß (12%)
Suffisant pour96 microplate wells
Contenus des kitsStop Solution, 6mL, TMB Substrate Solution, 12mL, Streptavidin-HRP Concentrate, 75µL, Standard Diluent, 12mL, Lyophilized Recombinant Mouse IL-1 beta Standard, 2 vials, HRP Dilution Buffer, 13mL, Biotinylated Antibody Reagent, 12mL, Anti-Mouse IL-1 beta Precoated 96-well Strip Plate, 1 ea, Adhesive Plate Covers, 6 ea, 20X Wash Buffer, 55mL
MesureAbsorbance at 450nm minus absorbance at 550nm
PrécisionIntra-assay CV ≤ 10%; inter-assay CV ≤ 10%
Quantité1 Plate Kit
Volume d’échantillon50 μL
CibleNatural and recombinant mouse interleukin-1 beta (IL-1ß)
Durée totale du dosage4 hr.
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