"/> Invitrogen™ Kit ELISA IL-8 humains | B-LABO France

Invitrogen™ Kit ELISA IL-8 humains

Réf. : BL-RE-097343Réf. fabricant : 10187573 5 Plate Kit
521,85 € HT

Interleukin 8 Recombinant protein SIL8 from Thermo Scientific™ specifically detects Interleukin 8 in Not Applicable samples, and it is validated for Control, ELISA and WB

Plage de dosage
25.6 to 1000pg/mL
Sensibilité du dosage
<2pg/mL
Description
Human IL-8 ELISA Reagent Kit
Format du kit d’immunoessai
Colorimetric ELISA (TMB substrate) with Plates
Type d’échantillon
Cell Culture Supernatant, Plasma (EDTA, Citrate, Heparin), Serum
Marque
Invitrogen

Assay IL-8 (human interleukin-8) levels in human serum, plasma (EDTA, heparin, sodium citrate), urine and culture supernatant samples with our Invitrgen™ Human IL-8 ELISA Kits.

These human IL-8 ELISA kits use pre-titered, matched pairs of coating and detection antibodies to achieve sensitive and accurate measurement of interleukin-8 (IL-8) in samples at 50 to 100μL per well. Also included are calibrated recombinant protein standard, streptavidin-HRP, TMB substrate solution and stop solution. The complete kits contain precoated 96-well strip plate(s) and lot-optimized reagents. The economically-priced reagent kit provides validated reagents only, requiring users to coat their own plates. The antibody-pair kit consists of the matched IL-8 antibody pairs and IL-8 standard only. All components undergo quality control testing to minimize lot-to-lot variability.

Assay range and other values listed above are for the pre-coated plate kit; see product documentation for values specific to the reagent kit and antibody-pair kit.

Includes:Complete Kits contain pre-coated antibody-plates, detection antibodies, buffers, diluents, standards, and substrates. The Reagent Kit (previously called a “Screening Set”) contains matched antibodies, standard and detection reagents but no coated plates. The Antibody Pair Kit (previously called a “MiniKit”) contains only matched antibodies and standard (no plates or detection reagents).

Caractéristiques principales

  • Target: Natural and recombinant human interleukin-8 (IL-8) and has been shown to recognize IL-8 derived from stimulated human umbilical vein endothelial cells
  • Format: Colorimetric ELISA (TMB substrate) in clear 96-well strip plate
  • Measurement: Absorbance at 450nm minus absorbance at 550nm
  • Assay Range: 25.6 to 1000pg/mL
  • Sensitivity: < 2pg/mL
  • Precision: Intra-assay CV < 10%; inter-assay CV < 10%
  • Sample Types: Culture supernatant, urine, serum, plasma (EDTA, sodium citrate and heparin)
  • Total Assay Time: 3 hours
  • Sample Size: 50μL
  • Specificity: No significant cross-reaction with with human PF-4, GROα, IL-1α, IL-1β, IL-2, IL-3, IL-4, IL-6, IL-7, IL-10, IL-12, TNFα, IFNα, IFNγ, or GM-CSF

Caractéristiques techniques

MarqueInvitrogen
Réf. B-LABOBL-RE-097343
Réf. fabricant10187573
Plage de dosage25.6 to 1000pg/mL
Sensibilité du dosage<2pg/mL
Format du kit d’immunoessaiColorimetric ELISA (TMB substrate) with Plates
Type d’échantillonCell Culture Supernatant, Plasma (EDTA, Citrate, Heparin), Serum
SpécificitéNo significant cross-reaction with with human PF-4, GROa, IL-1a, IL-1ß, IL-2, IL-3, IL-4, IL-6, IL-7, IL-10, IL-12, TNFa, IFNa, IFNg, or GM-CSF
Suffisant pour5 x 96 microplate wells
Contenus des kitsIL-8 Detection Antibody, 1 vial, Human IL-8 Standard, 5 vials, Stop Solution, 55mL, Streptavidin-HRP Conjugate, 0.25mL, TMB Substrate Solution, 55mL, IL-8 Coating Antibody, 1 vial
MesureAbsorbance at 450nm minus absorbance at 550nm
PrécisionIntra-assay CV <10%; inter-assay CV <10%
Quantité5 Plate Kit
Volume d’échantillon50 μL
CibleNatural and recombinant human interleukin-8 (IL-8) and has been shown to recognize IL-8 derived from stimulated human umbilical vein endothelial cells
Durée totale du dosage3 hr.

Documentation

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