"/> Invitrogen™ Kit ELISA IL-12 (total) de souriss | B-LABO France

Invitrogen™ Kit ELISA IL-12 (total) de souriss

Réf. : BL-RE-097355Réf. fabricant : 10485335 1 Plate Kit
548,10 € HT

Measure levels of total interleukin-12 (p40 + p70) in mouse serum, plasma and culture supernatant samples using 96-well plates.

Plage de dosage
47 to 3000pg/mL
Sensibilité du dosage
<12pg/mL
Description
Mouse IL-12 Total (p70, p40) ELISA Kit
Format du kit d’immunoessai
Colorimetric ELISA (TMB substrate) with Plates
Type d’échantillon
Cell Culture Supernatant, Plasma (EDTA, Citrate, Heparin), Serum
Marque
Invitrogen

Assay IL-12 p40 + p70 (total mouse interleukin-12) levels in mouse serum, plasma (EDTA, heparin, sodium citrate) and culture supernatant samples with our Thermo Scientific Mouse IL-12 (total) ELISA Kits.

These mouse IL-12 ELISA kits use pre-titered, matched pairs of coating and detection antibodies to achieve sensitive and accurate measurement of both active (p70) and inactive (p40) forms of interleukin-12 (IL-12 total) in samples at 50μL per well. Also included are calibrated recombinant protein standard, streptavidin-HRP, TMB substrate solution and stop solution. The complete kits contain pre-coated 96-well strip plate(s) and lot-optimized reagents. All components undergo quality control testing to minimize lot-to-lot variability.

Includes:Complete kits contain pre-coated antibody-plates, detection antibodies, buffers, diluents, standards, and substrates.

Caractéristiques principales

  • Target: Total (p70 and p40) natural and recombinant mouse interleukin-12 (IL-12)
  • Format: Colorimetric ELISA (TMB substrate) in clear 96-well strip plate
  • Measurement: Absorbance at 450nm minus absorbance at 550nm
  • Assay Range: 47 to 3000pg/mL
  • Sensitivity: < 12pg/mL
  • Precision: Dependent upon sample type (generally, intra- and inter-assay CV < 10%)
  • Sample Types: Cell culture supernatant, serum, plasma (EDTA, sodium citrate and heparin)
  • Total Assay Time: 3 hours
  • Sample Size: 50μL
  • Specificity: No significant cross-reaction with mouse G-CSF, GM-CSF, IFNγ, IL-1α, IL-1β, IL-2, IL-3, IL-4, IL-5, IL-6, IL-7, IL-9, IL-10, IL-18, Eotaxin, MCP-1, MIP-1α, RANTES or TNFα, human IL-12, or pig IL-12

Caractéristiques techniques

MarqueInvitrogen
Réf. B-LABOBL-RE-097355
Réf. fabricant10485335
Plage de dosage47 to 3000pg/mL
Sensibilité du dosage<12pg/mL
Format du kit d’immunoessaiColorimetric ELISA (TMB substrate) with Plates
Type d’échantillonCell Culture Supernatant, Plasma (EDTA, Citrate, Heparin), Serum
SpécificitéNo significant cross-reaction with mouse G-CSF, GM-CSF, IFNg, IL-1a, IL-1ß, IL-2, IL-3, IL-4, IL-5, IL-6, IL-7, IL-9, IL-10, IL-18, Eotaxin, MCP-1, MIP-1a, RANTES or TNFa, human IL-12, or pig IL-12
Suffisant pour96 microplate wells
Contenus des kitsTMB Substrate Solution, 12mL, 30X Wash Buffer, 50mL, Adhesive Plate Covers, 6 ea, Anti-Mouse IL-12 Total Precoated 96-well Strip Plate, 1 ea, Biotinylated Antibody Reagent, 12mL, HRP Dilution Buffer, 13mL, Lyophilized Recombinant Mouse IL-12 Standard, 2 vials, Standard Diluent, 20mL, Streptavidin-HRP Concentrate, 75uL, Stop Solution, 13mL
MesureAbsorbance at 450nm minus absorbance at 550nm
PrécisionDependent upon sample type (generally, intra- and inter-assay CV <10%)
Quantité1 Plate Kit
CibleTotal (p70 and p40) natural and recombinant mouse interleukin-12 (IL-12)
Durée totale du dosage3 hr.
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