Measure levels of mouse interleukin-2 in mouse serum and cell culture supernatant samples using 96-well plates.
Assay IL-2 (mouse interleukin-2) levels in mouse serum and culture supernatant samples with our Thermo Scientific™ Mouse IL-2 ELISA Kits.
These mouse IL-2 ELISA kits use pre-titered, matched pairs of coating and detection antibodies to achieve sensitive and accurate measurement of interleukin-2 (IL-2) in samples at 50 to 100μL per well. Also included are calibrated recombinant protein standard, TMB substrate solution and stop solution. The complete kits contain pre-coated 96-well strip plate(s) and lot-optimized reagents. The antibody-pair kit consists of the matched IL-2 antibody pairs and IL-2 standard only. All components undergo quality control testing to minimize lot-to-lot variability.
Assay range and other values listed above are for the pre-coated plate kit; see product documentation for values specific to the antibody-pair kit.
Includes:Complete Kits contain pre-coated antibody-plates, detection antibodies, buffers, diluents, standards, and substrates. The Antibody Pair Kit (previously called a “MiniKit”) contains only matched antibodies and standard (no plates or detection reagents).
| Marque | Invitrogen |
|---|---|
| Réf. B-LABO | BL-RE-097356 |
| Réf. fabricant | 10300974 |
| Plage de dosage | 34 to 850pg/mL |
| Sensibilité du dosage | <3pg/mL |
| Format du kit d’immunoessai | Colorimetric ELISA (TMB substrate) with Plates |
| Type d’échantillon | Cell Culture Supernatant, Serum |
| Spécificité | No significant cross-reaction with mouse IL-3, IL-4,IL-5, IL-6, IL-10, IFNg, GM-CSF, TNFa or human IL-2 |
| Suffisant pour | 96 microplate wells |
| Contenus des kits | TMB Substrate Solution, 13mL, Stop Solution, 13mL, Anti-Mouse IL-2 Precoated 96-well Strip Plate, 1 ea, Standard Diluent, 12mL, Plate Reagent, 12mL, Conjugate Dilution Buffer, 14mL, Anti-IL2-HRP Conjugate Concentrate, 0.10mL, Adhesive Plate Covers, 6 ea, 30X Wash Buffer, 50mL, Lyophilized Recombinant Mouse IL-2 Standard, 2 vials |
| Mesure | Absorbance at 450nm minus absorbance at 550nm |
| Précision | Intra-assay CV <10%; inter-assay CV <10% |
| Quantité | 1 Plate Kit |
| Volume d’échantillon | 50 μL |
| Cible | Natural and recombinant mouse interleukin-2 (IL-2) |
| Durée totale du dosage | 3.5 hr. |
