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Invitrogen™ Novex™ Thermo Scientific™ Human IL-2R ELISA Kit

Réf. : BL-RE-097357Réf. fabricant : 10485345 1 Plate Kit
535,50 € HT

Desiged to assay sIL-2R (human soluble interleukin-2 receptor; also called CD25) levels in human serum, plasma (with EDTA, heparin, sodium citrate), and cell culture supernatant samples

Numéro d’adhésion
NP_000408.1, NM_000417.2
À utiliser avec (application)
Chromogenic Detection
Identification génétique (Entrez)
3559
Contenus des kits
13 mL stop solution, 13 mL TMB substrate solution, 55 mL 20X wash buffer, 6 mL conjugate reagent, Adhesive plate covers, 2 each, anti-Human sIL-2R Precoated 96-well Strip Plate, 1 each, Lyophilized recombinant Human sIL-2R Standard, 2 vials, Sample diluent, 6 mL
Quantité
1 Plate Kit
Marque
Invitrogen

This kit uses pre-titered, matched pairs of coating and detection antibodies to achieve sensitive and accurate measurement of sIL-2R levels in samples. Complete kit includes one pre-coated 96-well strip plate, lot-optimized reagents, calibrated recombinant protein standard, TMB substrate solution, and stop solution. All components undergo quality control testing to minimize lot-to-lot variability. Kit is an alternate product for Cat. No. KHR0021.

Standards are calibrated to an internal sIL-2R reference preparation of supernatant from a transfected cell line that secretes recombinant soluble IL-2R. We define 1000 units as the amount of sIL-2R present in 1mL of an internal reference preparation. Units/mL can be converted to sIL-2R pg/mL using the following formula: (sIL-2R pg/mL) = (sIL-2R U/mL) x (1/0.113).

Protein Quantitation, Proteins, Expression, Isolation & Analysis

Points forts

Informations sur la commande

Shipping Condition: Room Temperature

Caractéristiques principales

  • Target: natural and recombinant human soluble interleukin-2 receptor (sIL-2R), also called CD25
  • Assay range: 0 to 6500U/mL
  • Sensitivity: < 24U/mL
  • Sample types: cell culture supernatant, serum, plasma (with EDTA, sodium citrate, heparin)
  • Total assay time: 2.5 hours
  • Sample size: 50μL
  • Specificity: no significant cross-reaction with human IL-1α, IL-1β, IL-2, IL-3, IL-4, IL-5, IL-6, IL-7, IL-8, IL-9, IL-10, IL-11, IL-12, IL-13, IL-15, GM-CSF, IFNγ, IFNα, TNFβ, or mouse IL-2

Caractéristiques techniques

MarqueInvitrogen
Réf. B-LABOBL-RE-097357
Réf. fabricant10485345
Numéro d’adhésionNP_000408.1, NM_000417.2
À utiliser avec (application)Chromogenic Detection
Identification génétique (Entrez)3559
Contenus des kits13 mL stop solution, 13 mL TMB substrate solution, 55 mL 20X wash buffer, 6 mL conjugate reagent, Adhesive plate covers, 2 each, anti-Human sIL-2R Precoated 96-well Strip Plate, 1 each, Lyophilized recombinant Human sIL-2R Standard, 2 vials, Sample diluent, 6 mL
Quantité1 Plate Kit
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