Assay IL-1aα (mouse interleukin-1 alpha) levels in mouse serum, plasma (EDTA, heparin, sodium citrate)and culture supernatant samples with our Thermo Scientific Mouse IL-1 alpha ELISA Kits.
These mouse IL-1 alpha ELISA kits use pre-titered, matched pairs of coating and detection antibodies to achieve sensitive and accurate measurement of interleukin-1 alpha (IL-1α) in samples at 50 per well. Also included are calibrated recombinant protein standard, streptavidin-HRP, TMB substrate solution and stop solution. The complete kits contain precoated 96-well strip plate(s) and lot-optimized reagents. All components undergo quality control testing to minimize lot-to-lot variability.
Includes:Complete kits contain pre-coated antibody-plates, detection antibodies, buffers, diluents, standards, and substrates.
Caractéristiques principales
Target: Natural and recombinant mouse interleukin-1 alpha (IL-1α) Format: Colorimetric ELISA (TMB substrate) in clear 96-well strip plate Measurement: Absorbance at 450nm minus absorbance at 550nm Assay Range: 15.6 to 1000pg/mL Sensitivity: < 6pg/mL Precision: Intra-assay CV ≤ 10%; Inter-assay CV ≤ 10% Sample Types: Cell culture supernatant, serum, plasma (EDTA, sodium citrate and heparin) Total Assay Time: 3 hours Sample Size: 50μL Specificity: No significant cross-reaction with human IL-1α, human IL-1β, or mouse IL-1β, IL-3, IL-4, IL-5, IL-7, IL-10, TNFα, IFNγ, or GM-CSF Caractéristiques techniques
Marque Invitrogen Réf. B-LABO BL-RE-097417 Réf. fabricant 10209193 Plage de dosage 15.6 to 1000pg/mL Sensibilité du dosage <6pg/mL Format du kit d’immunoessai Colorimetric ELISA (TMB substrate) with Plates Type d’échantillon Cell Culture Supernatant, Plasma (EDTA, Citrate, Heparin), Serum Spécificité No significant cross-reaction with human IL-1a, human IL-1ß, or mouse IL-1ß, IL-3, IL-4, IL-5, IL-7, IL-10, TNFa, IFNg, or GM-CSF Suffisant pour 96 microplate wells Contenus des kits Biotinylated Antibody Reagent, 8mL, Anti-Mouse IL-1 alpha Precoated 96-well Strip Plate, 1 ea, Adhesive Plate Covers, 6 ea, 30X Wash Buffer, 50mL, HRP Dilution Buffer, 14mL, Streptavidin-HRP Concentrate, 75µL, Lyophilized Recombinant Mouse IL-1 alpha Standard, 2 vials, TMB Substrate Solution, 13mL, Stop Solution, 13mL, Standard Diluent, 12mL Mesure Absorbance at 450nm minus absorbance at 550nm Précision Intra-assay CV ≤ 10%; Inter-assay CV ≤ 10% Quantité 1 Plate Kit Volume d’échantillon 50 μL Cible Natural and recombinant mouse interleukin-1 alpha (IL-1a) Durée totale du dosage 3 hr.
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