Pour la détection quantitative du granzyme humain B.
The Human Granzyme B ELISA quantitates Hu Granzyme B in human serum or cell culture medium. The assay will exclusively recognize both natural and recombinant Hu Granzyme B. Principle of the method The Human Granzyme B solid-phase sandwich ELISA (enzyme-linked immunosorbent assay) is designed to measure the amount of the target bound between a matched antibody pair. A target-specific antibody has been pre-coated in the wells of the supplied microplate. Samples, standards, or controls are then added into these wells and bind to the immobilized (capture) antibody. The sandwich is formed by the addition of the second (detector) antibody, a substrate solution is added that reacts with the enzyme-antibody-target complex to produce measurable signal. The intensity of this signal is directly proportional to the concentration of target present in the original specimen. Rigorous validation Each manufactured lot of this ELISA kit is quality tested for criteria such as sensitivity, specificity, precision, and lot-to-lot consistency. See manual for more information on validation.
| Marque | Invitrogen |
|---|---|
| Réf. B-LABO | BL-RE-097475 |
| Réf. fabricant | 15551517 |
| Numéro d’adhésion | P10144 |
| Plage de dosage | 0,66-480 pg/ml |
| Sensibilité du dosage | 0,2 pg/ml |
| Conjugué | Biotine |
| Type de produit | Kit ELISA |
| Type d’échantillon | Sérum, Surnageant |
| Espèces cibles | Humaine |
| À utiliser avec (application) | ELISA |
| À utiliser avec (équipement) | Lecteur de microplaques colorimétriques |
| Identification génétique (Entrez) | 3002 |
| Symbole de gène(s) | C11,CCPI,CGL-1,CGL1,CSP-B,CSPB,CTLA1,CTSGL1,HLP,SECT |
| Coefficient de variation inter-dosage | 10.4% |
| Coefficient de variation intra-dosage | 8.5% |
| Contenus des kits | Plaque à 96 puits pré-enduite, Étalon, Diluant d’échantillon, Concentrat de tampon de dosage, Anticorps de détection biotinylé, Tampon de lavage, Chromogène, Solution d’arrêt, Couvercles de plaques adhésives, Adhesive Plate Covers |
| Marqueur ou colorant | HRP |
| Quantité | 10 x 96 tests |
| État réglementaire | RUO |
| Volume d’échantillon | Sérum, 50 μl ; surnageant, 50 μl |
| Conditions de stockage | 2°C à 8°C |
| Synonyme | Granzyme2, Fragmentin2, protéine lymphocytaire humaine |
| Cible | Humaine |
| Heure du test | 1 h 20 min |
| Durée totale du dosage | 2 h 45 min |
