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Invitrogen™ Dynabeads™ SARS-CoV-2 Spike IgG ELISA Kit

Réf. : BL-RE-097490Réf. fabricant : 17797433 5 x 96 reactions
2 195,00 € HT

Le kit ELISA pour IgG anti-Spike SARS-CoV-2 Dynabeads™ est un essai immuno-enzymatique (ELISA) en sandwich en phase solide conçu pour détecter et quantifier le taux d’IgG humaines anti-SARS-CoV-2 dans les échantillons de sérum et de plasma.Ce produit peut être utilisé à la fois pour des dosages qualitatifs et quantitatifs.

Certifications/Conformité
ISO 9001
Contenu et stockage
Kit contains 5X of the following reagents:
• Dynabeads™ SARS-CoV-2 Spike, 3 mL (1 mg/mL)
• Human SARS-CoV-2 IgG High Control antibody, 1 vial (lyophilized)
• Human SARS-CoV-2 IgG Low Control antibody, 1 vial (lyophilized)
• Human SARS-CoV-2 IgG Calibrator (medium control) antibody, 1 vial (lyophilized)
• Wash buffer (20X concentrated), 50 mL
• Assay buffer (20X concentrated), 50 mL
• Substrate solution (tetramethylbenzidine), 15 mL
• Stop solution, 15 mL
• Human SARS-CoV-2 IgG HRP Conjugate, 0.12 mL

Store at 2–8°C.

Méthode de détection
Absorbance at 450 nm
Type de produit
SARS-CoV-2 ELISA IgG Kit
À utiliser avec (application)
Binding of human IgG towards SARS-CoV-2 (Spike trimer)
Marque
Invitrogen

Caractéristiques principales

  • A solid‐phase sandwich enzyme‐linked immunosorbent assay (ELISA) designed to detect and quantify the level of human SARS-CoV-2 IgG from serum and plasma samples
  • The steps required for use are simply to pre-dilute the reagents, fill the wells of the KingFisher plastic plates, and push start on the instrument
  • After the run, the sample is transferred to a plate compatible with the absorbance reader and the OD is read at 450 nm
  • A DynaMag-96 well magnet can be used while still taking advantage of the faster binding kinetics of magnetic beads compared to the more traditional plate-bound ELISAs
  • Kit contains all buffers required, as well as calibrator antibodies, HRP-conjugated detection antibodies, substrate solution, beads, and stop solution sufficient for one 96-well plate
  • The intra-assay CV value is typically < 5% (range 1.4-4.3%), while the inter-assay CV is < 9% (range 3.8-8.1%)
  • Assay can be used both for qualitative and quantitative assays
  • One of the fastest ELISA assays for SARS-CoV-2 serology
  • Dynabeads facilitated automation limits hands-on time
  • For research use only, not for use in diagnostic procedures

Caractéristiques techniques

MarqueInvitrogen
Réf. B-LABOBL-RE-097490
Réf. fabricant17797433
Certifications/ConformitéISO 9001
Contenu et stockageKit contains 5X of the following reagents:<br />• Dynabeads™ SARS-CoV-2 Spike, 3 mL (1 mg/mL)<br />• Human SARS-CoV-2 IgG High Control antibody, 1 vial (lyophilized)<br />• Human SARS-CoV-2 IgG Low Control antibody, 1 vial (lyophilized)<br />• Human SARS-CoV-2 IgG Calibrator (medium control) antibody, 1 vial (lyophilized)<br />• Wash buffer (20X concentrated), 50 mL<br />• Assay buffer (20X concentrated), 50 mL<br />• Substrate solution (tetramethylbenzidine), 15 mL<br />• Stop solution, 15 mL<br />• Human SARS-CoV-2 IgG HRP Conjugate, 0.12 mL<br /><br>Store at 2–8°C.<br><br>
Méthode de détectionAbsorbance at 450 nm
Type de produitSARS-CoV-2 ELISA IgG Kit
À utiliser avec (application)Binding of human IgG towards SARS-CoV-2 (Spike trimer)
À utiliser avec (équipement)KingFisher™ Flex or Apex Instruments, DynaMag™-96 Side Skirted, Magnetic 96-Well Separator, ELISA Plate Reader (450 nm)
Quantité5 x 96 reactions
Conditions d’expéditionWet ice (2° to 8°C)
Diamètre (métrique) des microbilles2.7 μm
MatériauMonosized, superparamagnetic polystyrene magnetic beads
Compatibilité à haut débitYes, on KingFisher™ Flex or Apex instruments
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