The Pierce BCA Protein Assay Kit is a high-precision, detergent-compatible protein assay for determination of protein concentration. Pierce BCA reagents provide accurate determination of protein concentration with most sample types encountered in protein research.
Concentration
BSA: 2 mg/mL
Contenu et stockage
Sufficient For: 50 tube assays or 500 microplate assays • BCA Reagent A, 100 mL • BCA Reagent B, 2.5 mL • Albumin Standard, 2 mg/mL, 2 mL
Store at room temperature. Store BSA Protein Standards at 4°C after opening.
Description
Pierce BCA Protein Assay Kit, 100 mL
Doser
BCA Assay
Méthode de détection
Colorimetric
Marque
Thermo Scientific
Kits are available with or without Pierce Dilution-Free BSA Protein Standards, which are a set of seven pre-diluted BSA standards, packaged in a multichannel pipette-friendly tubestrip. The tubestrip includes a single empty tube that enables users to add their own sample buffer for the purpose of blank subtraction.
Kit options are available that include Pierce Dilution-Free BSA Protein Standards, which are a set of seven pre-diluted BSA standards, packaged in a multichannel pipette-friendly tubestrip. The tubestrip includes a single empty tube that enables users to add their own sample buffer for the purpose of blank subtraction.
Features of the BCA Protein Assay Kit include:
Points forts
How the assay works
The BCA Protein Assay combines the well-known reduction of Cu2+ to Cu1+ by protein in an alkaline medium with the highly sensitive and selective colorimetric detection of the cuprous cation (Cu1+) by bicinchoninic acid (BCA). The first step is the chelation of copper with protein in an alkaline environment to form a light blue complex. In this reaction, known as the biuret reaction, peptides containing three or more amino acid residues form a colored chelate complex with cupric ions in an alkaline environment containing sodium potassium tartrate.
In the second step of the color development reaction, BCA reacts with the reduced (cuprous) cation that was formed in step one. The intense purple-colored reaction product results from the chelation of two molecules of BCA with one cuprous ion. The BCA/copper complex is water soluble and exhibits a strong linear absorbance at 562 nm with increasing protein concentrations. The complex is approximately 100 times more sensitive (lower limit of detection) than the pale blue color of the first reaction.
Caractéristiques principales
Colorimetric—estimate visually or measure with a standard spectrophotometer or plate reader at 562 nm
Accurate—exhibits half the protein-to-protein variation observed with dye-binding methods (Bradford)
Compatible—unaffected by typical concentrations of most ionic and non-ionic detergents
Short assay time—30-min incubation; much easier and four times faster than classical Lowry methods
Wide assay range—linear working range for BSA of 20 to 2000 μg/mL
Sensitive—detect down to 5 μg/mL with enhanced protocol
No more serial dilutions—kit options include Dilution-Free BSA Protein Standards which eliminate the need to perform serial dilutions when generating a standard curve
Caractéristiques techniques
Marque
Thermo Scientific
Réf. B-LABO
BL-RE-101826
Réf. fabricant
18064090
Concentration
BSA: 2 mg/mL
Contenu et stockage
Sufficient For: 50 tube assays or 500 microplate assays<br>• BCA Reagent A, 100 mL<br>• BCA Reagent B, 2.5 mL<br>• Albumin Standard, 2 mg/mL, 2 mL<br><br>Store at room temperature. Store BSA Protein Standards at 4°C after opening.
Doser
BCA Assay
Méthode de détection
Colorimetric
Gamme de produits
Pierce™
Type de produit
Protein Quantitation Assay
Spécificité
Not Target-Specific
Suffisant pour
50 Tube Assays or 500 Microplate Assays
À utiliser avec (application)
Solution-based Detection, Absorbance
À utiliser avec (équipement)
Spectrophotometer, Microplate Reader
Contenus des kits
2 mL vial
Quantité
1 Kit (100 mL)
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