The Human IL-6 Magnetic Bead Kit for the Luminex™ platform is specifically designed for quantification of low levels of human IL-6 in serum, plasma, and tissue culture supernatant samples. By mixing this kit with other targets from our human magnetic kit portfolio, one can obtain more data from each sample, saving both money and time compared to more traditional methods (such as ELISA). This kit is recommended to be run with the Human Cytokine Magnetic Buffer Reagent Kit (Cat. No. LHB0001M). This assay employs magnetic beads, facilitating automation, decreasing hands-on time, and increasing throughput and precision. The assay is suitable for use with the Luminex™ 200, FLEXMAP 3D™, and MAGPIX™ systems.
Superior performance—accurate, reproducible, and sensitive quantitation of multiple proteins
High quality—fully qualified antibodies produce excellent specificity and sensitivity
Fast and easy protocols—perform your assays and analyze your data typically in less than one day Luminex xMAP technology—effective and proven analytical tool The Human IL-6 Magnetic Bead Kit is based on xMAP™ technology. The use of a suspension bead-based technology enables the multiplexing capabilities of the Luminex assays. Magnetic microspheres are internally dyed with red and infrared fluorophores of differing intensities. Each bead has a unique number, or bead region, allowing differentiation of one bead from another. Beads covalently bound to different antibodies can be mixed in the same assay. Upon completion of the sandwich immunoassay, magnetic beads must be measured using a Luminex™ detection system (MAGPIX™, 100™/200™, or FLEXMAP 3D™). The instrument uses xPONENT™ software to distinguish bead color (analyte) and R-PE fluorescence intensity (assay signal strength) to quantify target(s). Magnetic beads let you do more with your time Magnetic bead-based assays enable automation, decrease hands-on time, and increase throughput and precision. Using the fast and easy Luminex protocols, you can obtain results in as little as 3.5 hours. This line of assays was carefully designed and tested for maximum sensitivity, range, and correlation. Using the same components as our polystyrene bead-based assays allows for performance with identical quality and consistency. Standard curves generated with this assay system extend over several orders of magnitude of reagent concentration, while the sensitivity and quality of the assays are comparable to ELISAs and our polystyrene bead-based assays, enabling superior performance. More validation for confidence in your results The Human IL-6 Magnetic Bead Kit has been validated using the same rigorous criteria that distinguishes Luminex assays from other commercially available assays. Our fully qualified antibodies help ensure the specificity and sensitivity of our assays. Every singleplex kit and premixed multiplex kit comes with a product insert that outlines the specifications for each marker in the assay.Learn more about all Invitrogen™ assays for the Luminex™ platform.
Caractéristiques techniques
| Marque | Invitrogen |
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| Réf. B-LABO | BL-RE-108307 |
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| Réf. fabricant | 15461164 |
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| Type de produit | ELISA Kit |
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| Quantité | 100 Tests |
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| Espèces | Human |
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| Méthode de détection | Fluorescent |
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| Conjugué | R-PE |
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| Type de microbilles | Magnetic |
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| À utiliser avec (application) | Protein biology, luminex assays and other bead-based assays, protein assays and analysis |
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| À utiliser avec (équipement) | Luminex Instruments |
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| Identification génétique (Entrez) | 3569 |
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| Symbole de gène(s) | IL6 |
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| Type de substrat | Cytokines & Receptors |
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| Contenus des kits | Antibody-coated capture beads, standard, detector antibody and lot-specific technical data sheet |
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| Contenu et stockage | 2°C to 8°C |
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| État réglementaire | For research use only (RUO) |
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