Alexa Fluor™ 488 Hydroxylamine is useful as a cell tracer and as a reactive dye for labeling aldehydes or ketones in polysaccharides or glycoproteins.
Description
Hydroxylamine 488 Alexa Fluor™, 1 mg, jaune, solide, formule moléculaire C27H38N4O8S, poids moléculaire 578,68, marquage 488 Alexa Fluor™, aldéhyde, acide carboxylique, réactivité chimique des cétones, longueur d’onde d’excitation / émission 494/518 nm, utilisée pour le marquage
Formule
Solide
À utiliser avec (application)
Viabilité, prolifération et fonction cellulaires, étiquetage chimique, étiquetage des protéines et couplage
Quantité
1 mg
Type de produit
Hydroxylamine
Marque
Invitrogen
Detailed information about this AlexaFluor™ Hydroxylamine:
Points forts
Cell Tracking and Tracing Applications
Alexa Fluor™ hydrazides and Hydroxylamines are useful as low molecular weight, membrane-impermeant, aldehyde-fixable cell tracers, exhibiting brighter fluorescence and greater photostability than cell tracers derived from other spectrally similar fluorophores. They are easily loaded into cells by microinjection, infusion from patch pipette, or uptake induced by our Influx™ Pinocytic Cell-Loading Reagent (Cat. No. I14402).
Glycoprotein and Polysaccharide Labeling Applications
The Alexa Fluor™ hydrazides and Hydroxylamines are reactive molecules that can be used to add a fluorescent label to biomolecules containing aldehydes or ketones. Aldehydes and ketones can be introduced into polysaccharides and glycoproteins by periodate-mediated oxidation of vicinal diols. Galactose oxidase can also be used to oxidize terminal galactose residues of glycoproteins to aldehydes.
Hydrazide vs Hydroxylamine
Hydrazine derivatives react with ketones and aldehydes to yield relatively stable hydrazones. Hydroxylamine derivatives (aminooxy compounds) react with aldehydes and ketones to yield oximes. Oximes are superior to hydrazones with respect to hydrolytic stability. Both hydrazones and oximes can be reduced with sodium borohydride (NaBH4) to further increase the stability of the linkage.
Learn More About Protein and Antibody Labeling
We offer a wide selection of Molecular Probes™ antibody and protein labeling kits to fit your starting material and your experimental setup. See our Antibody Labeling kits or use our Labeling Chemistry Selection Tool for other choices. To learn more about our labeling kits, read Kits for Labeling Proteins and Nucleic Acids—Section 1.2 in The Molecular Probes™ Handbook.
We'll Make a Custom Conjugate for You
If you can't find what you're looking for in our online catalog, we'll prepare a custom antibody or protein conjugate for you. Our custom conjugation service is efficient and confidential, and we stand by the quality of our work. We are ISO 13485:2000 certified.
Related Products
DMSO (dimethylsulfoxide) (Cat. No. D12345)
Antibody Conjugate Purification Kit for 0.5-1 mg (A33086)
Antibody Conjugate Purification Kit for 20-50 μg (A33087)
Antibody Conjugate Purification kit for 50-100 μg (A33088)
Caractéristiques principales
Fluorophore label: Alexa Fluor™ 488 dye
Reactive group: Hydroxylamine
Reactivity: Aldehydes or ketones
Ex/Em of the conjugate: 494/518 nm
Extinction coefficient: 77,000 cm-1M-1
Spectrally similar dyes: FITC, GFP
Molecular weight: 895.07
Caractéristiques techniques
Marque
Invitrogen
Réf. B-LABO
BL-RE-129727
Réf. fabricant
10226602
Formule
Solide
À utiliser avec (application)
Viabilité, prolifération et fonction cellulaires, étiquetage chimique, étiquetage des protéines et couplage
Quantité
1 mg
Type de produit
Hydroxylamine
Plage de pH
4 à 10
Poids moléculaire
578,68 kDa
Pureté
HPLC, 99 % à 254 nm
Contenu et stockage
-5°C à -30°C
Réactivité chimique
Acide carboxylique, cétone, aldéhyde
Conditions d’expédition
Room Temperature
Groupement de réactifs
Amine, hydroxylamine
Marqueur ou colorant
Alexa Fluor™ 488
Type d’étiquette
Colorants Alexa Fluor
Couleur
Jaune
Gamme de produits
Alexa Fluor™
Émission
518
Excitation
494 nm
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