The Mouse SYB16 (CXCL16) ProcartaPlex Simplex Kit measures SYB16 (CXCL16) protein and is designed to be combinable with other Simplex kits so that you can create your own multiplex panel that utilizes Luminex xMAP technology for protein detection/quantitation.
Type de produit
Kit simplex
Quantité
96 dosages
Espèces
Mouse
Combinability
Combinable
Type de microbilles
Magnetic
Marque
Invitrogen
ProcartaPlex immunoassays are based on the principles of a sandwich ELISA, using two highly specific antibodies binding to different epitopes of one protein to quantitate up to 80 protein targets simultaneously when using the FLEXMAP 3D or Luminex 200 instrument and up to 50 protein targets when using the MAGPIX instrument. ProcartaPlex assays require as little as 25 μL of plasma or serum, or 50 μL of cell culture supernatant, and just four hours to obtain analyzed results.
The Luminex MagPlex superparamagnetic microsphere beads in the ProcartaPlex assay are internally dyed with precise proportions of red and infrared fluorophores to create 100 spectrally unique signatures that can be identified by the Luminex xMAP detection systems (Luminex 200, FLEXMAP 3D, and MAGPIX systems). Similar to a sandwich ELISA, the ProcartaPlex assay uses matched antibody pairs to identify the protein of interest. In a ProcartaPlex multiplex assay, each spectrally unique bead is labeled with antibodies specific for a single target protein, and bound proteins are identified with biotinylated antibodies and streptavidin–R-phycoerythrin (RPE). The conjugation of protein-specific antibodies to a distinct bead allows for analysis of multiple targets in a single well.
The most significant difference between a ProcartaPlex assay and ELISA is that the capture antibody in the ProcartaPlex assay is conjugated to a magnetic bead and not adsorbed to the microplate well, so the ProcartaPlex assay reagents are free-floating in the solution. For detection, the Luminex 200 instrument, for example, contains two lasers, one to distinguish the spectral signature of each bead and the second to quantify the amount of RPE fluorescence, which is proportional to the amount of protein present in the sample. ProcartaPlex multiplex assays can profile up to 80 times more target proteins using significantly less sample in the same time that it takes to perform a traditional sandwich ELISA.
ProcartaPlex Simplex kits provide the ability to create your own unique panel . More than 90% of ProcartaPlex Simplex targets can be combined, providing you with superior flexibility when creating your own multiplex panel.
Caractéristiques principales
Flexible panels—design your own panels with Simplex kits to measure your own array of targets
More results per sample—measure up to 80 protein targets in a single 25–50 μL sample
Well-established Luminex technology—the most referenced multiplexing platform for protein detection and quantitation
Caractéristiques techniques
Marque
Invitrogen
Réf. B-LABO
BL-RE-174903
Réf. fabricant
17867250
Type de produit
Kit simplex
Quantité
96 dosages
Espèces
Mouse
Combinability
Combinable
Type de microbilles
Magnetic
Zone des microbilles
SYB16 (CXCL16) [73]
Sensibilité du dosage
Voir le certificat d’analyse
Plage de dosage
Voir le certificat d’analyse
Format
Kit simplex
À utiliser avec (équipement)
Instruments Luminex™
Expression
CXCL16
Alias de gène
SCYB16, SR-PSOX, CXCLG16
Identification génétique (Entrez)
58191
Symbole de gène(s)
CXCL16
Coefficient de variation inter-dosage
Moins de 15%
Coefficient de variation intra-dosage
Moins de 15 %
Gamme de produits
ProcartaPlex™
Type d’échantillon
Serum, Plasma, Cell Culture Supernatants
Volume d’échantillon
Serum, Plasma: 25 μL, CCS: 50 μL
Synonyme
SCYB16, SR-PSOX, CXCLG16
Contenu et stockage
<p>• 1 vial Capture Beads (50X)<br />• 1 vial Biotinylated Detection Antibody (50X)<br />• 2 vials Mouse Standard Mix (lyophilized)<br /><br />Store at 2°C to 8°C.<br /><br /></p>
Conditions d’expédition
Wet Ice
Research Area
Immunology
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