The Non-Human Primate IL-7 Simplex ProcartaPlex Kit measures IL-7 protein and is designed to be combinable with other Simplex kits so that you can create your own multiplex panel that utilizes Luminex xMAP technology for protein detection/quantitation.
Type de produit
Kit simplex
Quantité
96 dosages
Espèces
Non-Human Primate
Combinability
Combinable
Zone des microbilles
IL-7 [26]
Marque
Invitrogen
ProcartaPlex immunoassays are based on the principles of a sandwich ELISA, using two highly specific antibodies binding to different epitopes of one protein to quantitate all protein targets simultaneously using a Luminex instrument. ProcartaPlex multiplex assays require as little as 25 μL of plasma or serum, or 50 μL of cell culture supernatant, and just four hours to obtain analyzed results.
ProcartaPlex assays utilize Luminex xMAP (multianalyte profiling) technology for the simultaneous detection and quantitation of up to 80 protein targets in a single 25–50 μL sample—from plasma, serum, cell culture supernatants, and other bodily fluids.
The Luminex beads in the ProcartaPlex assay are internally dyed with precise proportions of red and infrared fluorophores to create spectrally unique signatures that can be identified by the Luminex xMAP detection systems (e.g., Luminex 200, FLEXMAP 3D, and MAGPIX). Similar to a sandwich ELISA, the ProcartaPlex assay uses matched antibody pairs to identify the protein of interest. In a multiplexed assay, each spectrally unique bead is labeled with antibodies specific for a single target protein, and bound proteins are identified with biotinylated antibodies and streptavidin–R-phycoerythrin (RPE). The conjugation of protein-specific antibodies to a distinct bead allows for analysis of multiple targets in a single well.
The most significant difference between a ProcartaPlex assay and ELISA is that the capture antibody in the ProcartaPlex assay is conjugated to a bead and not adsorbed to the microplate well, so the ProcartaPlex assay reagents are free-floating in the solution. For detection, the Luminex 200 instrument, for example, contains two lasers, one to distinguish the spectral signature of each bead and the second to quantify the amount of RPE fluorescence, which is proportional to the amount of protein present in the sample. ProcartaPlex multiplex assays can profile more target proteins using significantly less sample in the same time that it takes to perform a traditional sandwich ELISA.
Caractéristiques principales
More results per sample—measure multiple protein targets simultaneously in a single 25–50 μL sample
Well-established Luminex technology—highly referenced multiplexing platform for protein detection and quantitation
Caractéristiques techniques
Marque
Invitrogen
Réf. B-LABO
BL-RE-174986
Réf. fabricant
17420743
Type de produit
Kit simplex
Quantité
96 dosages
Espèces
Non-Human Primate
Combinability
Combinable
Zone des microbilles
IL-7 [26]
Sensibilité du dosage
As determined for Lot 1: 0.2 pg/mL
Plage de dosage
As determined for Lot 1: 0.89 - 3650 pg/mL
Format
Kit simplex
À utiliser avec (équipement)
Instruments Luminex™
Expression
Interleukin 7
Identification génétique (Entrez)
574168
Symbole de gène(s)
IL7
Coefficient de variation inter-dosage
Moins de 15%
Coefficient de variation intra-dosage
Moins de 15 %
Gamme de produits
ProcartaPlex™
Protéine
Interleukin-7
Type d’échantillon
Serum
Volume d’échantillon
Serum, Plasma: 25 μL, CCS: 50 μL
ID UniProt
Q95J83
Contenu et stockage
• 1 vial Capture Beads (50X)<br />• 1 vial biotinylated Detection Antibody (50X)<br />• 2 vials NHP Standard Mix A (lyophilized)<br /><br />Store at 2-8°C.
