Invitrogen ProcartaPlex Human MAPK Pathway Panel (phospho), 6plex enables precise and simultaneous analysis of 6 phosphorylated proteins involved in the MAPK pathway.
The ProcartaPlex Human MAPK Pathway Panel (phospho), 6plex, enables simultaneous quantitation of six phosphorylated proteins included in the MAPK cell signaling pathway. It complements the ProcartaPlex Human MAPK Pathway Panel (total) 6plex (Cat. No. EPX080-97000-901) that measures the total protein level of the same eight proteins.
The panel is validated for use with cell lysates. For preparation of cell lysates, we recommend the use of either ProcartaPlex Cell Lysis Buffer (Cat. No. EPX-99999-000) or Cell Extraction Buffer (Cat. No. FNN0011) supplemented with 0.5 M EDTA and Halt Protease and Phosphatase Inhibitor Single to Use Cocktail 100X (Cat. No. 78440 or 78442). These buffers are not included in the panel. Depending on your cell system, other buffers can be suitable as well.
This kit is compatible with all Luminex instruments. If you use an xMAP INTELLIFLEX DR-SE instrument, we recommend our ProcartaPlex Human MAPK Pathway Dual Reporter Panel 2x6plex (Cat. No. EPX060-97217-DR), which enables the simultaneous quantitation of total and phosphorylated proteins on the same bead in a single well.
The panel is provided in a ready-to-use format with individual vials of capture and detection reagents formulated at 1X concentration, requiring less pipetting and experimental setup.
ProcartaPlex immunoassays are based on the principles of a sandwich ELISA, using two highly specific antibodies binding to different epitopes of one protein to quantitate all protein targets simultaneously using a Luminex instrument. ProcartaPlex multiplex assays require as little as 25 μL of plasma or serum, or 50 μL of cell culture supernatant, and just four hours to obtain analyzed results.
ProcartaPlex assays utilize Luminex xMAP (multianalyte profiling) technology for the simultaneous detection and quantitation of up to 80 protein targets in a single 25 to 50 μL sample from plasma, serum, cell culture supernatants, and other bodily fluids.
| Marque | Invitrogen |
|---|---|
| Réf. B-LABO | BL-RE-175242 |
| Réf. fabricant | 30276182 |
| Type de produit | Panneau multiplex |
| Quantité | 96 dosages |
| Espèces | Human |
| Combinability | Combinable |
| Type de microbilles | Magnetic |
| Zone des microbilles | JNK1/3[pT183/pT221] [13], p38[pT180/pY182] [14], FAK[pY397] [30], c-Myc[pS62] [53], ERK1[pT202/pY204] [62], MEK1[pS218/pS222] [63] |
| Sensibilité du dosage | Voir le certificat d’analyse |
| Plage de dosage | Voir le certificat d’analyse |
| À utiliser avec (équipement) | Instruments Luminex™ |
| Compatibilité à haut débit | Yes |
| Coefficient de variation inter-dosage | See Certificate of Analysis |
| Coefficient de variation intra-dosage | See Certificate of Analysis |
| Multiplex, taille Plex n° | 6plex |
| Gamme de produits | ProcartaPlex™ |
| Type d’échantillon | Cell lysates |
| Volume d’échantillon | Cell lysates (prediluted): 25 μL |
| Contenu et stockage | 2 vials Standard Mix (lyophilized)<br>1 vial Detection Antibody Mix (1X)<br>1 vial Bead Mix (1X)<br>1 bottle Universal Assay Buffer (10X)<br>1 bottle Streptavidin-PE (SA-PE) (1X)<br>1 bottle Wash Buffer (10X)<br>2 8-Tube Strip<br>1 Flat Bottom 96-well Plate, black<br>1 Microplate Lid<br>Plate Seals<br><br>Store as indicated on the Certificate of Analysis. |
| Conditions d’expédition | Wet Ice |
| Research Area | Signaling |
