Invitrogen ProcartaPlex Human c-Myc[pS62] Simplex Kit enables precise analysis of the Protein of Interest.
The ProcartaPlex Human c-Myc[pS62] Simplex Kit measures the level of phosphorylated c-Myc[pS62] in cell lysates. It is designed to be combinable with other Simplex kits so you can create your own multiplex panel that utilizes Luminex xMAP technology for protein quantitation. When combining multiple Simplex kits (i.e., when you are not using a pre-configured Multiplex Panel), only one buffer kit (sold separately) is needed for each assay plate regardless of plex size.
For preparation of cell lysates, we recommend the use of either ProcartaPlex Cell Lysis Buffer (Cat. No. EPX-99999-000) or Cell Extraction Buffer (Cat. No. FNN0011) supplemented with 0.5 M EDTA and Halt Protease and Phosphatase Inhibitor Single to Use Cocktail 100X (Cat. No. 78440 or 78442). These buffers are not included in the kit. Depending on your cell system, other buffers can be suitable as well.
The ProcartaPlex Human c-Myc[pS62] Simplex Kit is part of the ProcartaPlex Human MAPK Pathway Panel (phospho) 6plex (Cat. No. EPX060-97117-PHO), which complements the ProcartaPlex Human MAPK Pathway Panel (total) 6plex (Cat. No. EPX060-97017-901).
ProcartaPlex immunoassays are based on the principles of a sandwich ELISA, using two highly specific antibodies binding to different epitopes of one protein to quantitate all protein targets simultaneously using a Luminex instrument. ProcartaPlex multiplex assays require as little as 25 μL of plasma or serum, or 50 μL of cell culture supernatant, and just four hours to obtain analyzed results.
ProcartaPlex assays utilize Luminex xMAP (multianalyte profiling) technology for the simultaneous detection and quantitation of up to 80 protein targets in a single 25 to 50 μL sample from plasma, serum, cell culture supernatants, and other bodily fluids.
| Marque | Invitrogen |
|---|---|
| Réf. B-LABO | BL-RE-175247 |
| Réf. fabricant | 30275699 |
| Type de produit | Kit simplex |
| Quantité | 96 dosages |
| Espèces | Human |
| Combinability | Combinable |
| Type de microbilles | Magnetic |
| Zone des microbilles | c-Myc[pS62] [53] |
| Sensibilité du dosage | Voir le certificat d’analyse |
| Plage de dosage | Voir le certificat d’analyse |
| À utiliser avec (équipement) | Instruments Luminex™ |
| Expression | MYC |
| Alias de gène | c-Myc, v-myc |
| Identification génétique (Entrez) | 4609 |
| Symbole de gène(s) | MYC |
| Compatibilité à haut débit | Yes |
| Coefficient de variation inter-dosage | See Certificate of Analysis |
| Coefficient de variation intra-dosage | See Certificate of Analysis |
| Gamme de produits | ProcartaPlex™ |
| Type d’échantillon | Cell lysates |
| Volume d’échantillon | Cell lysates (prediluted): 25 μL |
| ID UniProt | P01106 |
| Synonyme | c-Myc, v-myc |
| Contenu et stockage | 1 vial Capture Beads (50x)<br>1 vial biotinylated Detection Antibody (50x)<br>2 vials Standard Mix (lyophilized)<br><br>Store as indicated on the Certificate of Analysis. |
| Conditions d’expédition | Wet Ice |
| Research Area | Signaling |
