pCLNDX expression vector is part of RetroMax system designed for maximal virus titer in 293 cells
Tampon de stockage
10µg in 20µL 1x TE (10mM Tris, pH 7.5, 1mM EDTA)
Contenu et stockage
Store at -20°C. Avoid freeze-thaw cycles.
Quantité
10 μg
Vecteur
pCLNDX Retrovirus Expression Vector
Marque
Novus Biologicals
The pCLNDX expression vector is a part of the RetroMax expression system and has been designed to maximize recombinant-retrovirus titers in a simple, efficient, and flexible experimental system. All members of the RetroMax expression vector family (pCLXSN, pCLNCX, pCLNRX, and pCLNDX) have an extended packaging signal (omega+) and are derived from safety modified retrovirus vectors in which the gag open reading frame has been stopped by a point mutation, thereby minimizing the opportunity for replication competent retrovirus production by recombination with packaging genome.
Points forts
Recommended Storage
Store at -20°C. Avoid freeze-thaw cycles.
Caractéristiques principales
RetroMax system takes advantages of two properties of 293 cells (high level of transfectibility and strong E1A-mediated stimulation of CMV promoter controlled transcription)
293 cells of nonmurine origin avoid problem of selective packaging and transfer of VL30 genomes (present in all murine packaging cells)
Vector supernatants are free of helper virus and are of sufficiently high titer within 2 days of transient transfection in 293 cells
For amplification, transform DH-5alpha or similar bacteria and plate on LB plates containing 50μg/mL ampicillin
Buffer: 10μg in 20μL 1x TE (10mM Tris, pH 7.5, 1mM EDTA)
Caractéristiques techniques
Marque
Novus Biologicals
Réf. B-LABO
BL-RE-090225
Réf. fabricant
18184724
Tampon de stockage
10µg in 20µL 1x TE (10mM Tris, pH 7.5, 1mM EDTA)
Contenu et stockage
Store at -20°C. Avoid freeze-thaw cycles.
Quantité
10 μg
Vecteur
pCLNDX Retrovirus Expression Vector
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