The ProcartaPlex Human Akt[pS473] Simplex Kit measures the phosphorylated Akt (phosphorylation site: Ser473) in cell lysates. It is designed to be combinable with other Simplex kits so you can create your own multiplex panel that utilizes Luminex xMAP technology for protein detection/quantitation.
Type de produit
Simplex Kit
Quantité
96 Tests
Espèces
Human
Combinability
Combinable
Type de microbilles
Magnetic
Marque
Invitrogen
For preparation of cell lysates, we recommend use of either ProcartaPlex Cell Lysis Buffer (Cat. No. EPX-999999-901) or Cell Extraction Buffer (Cat. No. FNN0011) supplemented with 0.5 M EDTA and Halt Protease and Phosphatase Inhibitor Single-Use Cocktail (100X) (Cat. No. 78440). These buffers are not included with the panel. Depending on your cell system, other buffers may be suitable as well.
Points forts
About ProcartaPlex Assays for the Luminex Platform
ProcartaPlex immunoassays are based on the principles of a sandwich ELISA, using two highly specific antibodies binding to different epitopes of one protein to quantitate all protein targets simultaneously using a Luminex instrument. ProcartaPlex multiplex assays require as little as 25 μL of plasma or serum or 50 μL of cell culture supernatant and just four hours to obtain analyzed results.
ProcartaPlex assays utilize Luminex xMAP (multianalyte profiling) technology for the simultaneous detection and quantitation of up to 80 protein targets in a single 25–50 μL sample from plasma, serum, cell culture supernatant, cell lysate, and other bodily fluids.
More results per sample—measure multiple protein targets simultaneously in a single 25–50 μL sample
Well-established Luminex technology—highly referenced multiplexing platform for protein detection and quantitation
Caractéristiques techniques
Marque
Invitrogen
Réf. B-LABO
BL-RE-097519
Réf. fabricant
18426718
Type de produit
Simplex Kit
Quantité
96 Tests
Espèces
Human
Combinability
Combinable
Type de microbilles
Magnetic
Sensibilité du dosage
As determined for Lot 1: 0.28 U/mL
Plage de dosage
As determined for Lot 1: 1.22 to 5000 U/mL
Format
Simplex Kit
À utiliser avec (équipement)
Luminex™ Instruments
Expression
Akt
Alias de gène
Akt, PKB, Protein kinase B
Identification génétique (Entrez)
207
Symbole de gène(s)
Akt
Coefficient de variation inter-dosage
As determined for Lot 1: 8.75%
Coefficient de variation intra-dosage
As determined for Lot 1: 4.16%
Gamme de produits
ProcartaPlex™
Type d’échantillon
Cell lysates
Volume d’échantillon
Cell lysates (prediluted): 25 μL
ID UniProt
P31749
Conditions d’expédition
Wet Ice
dimResearchArea
Signaling
Une question, une configuration particulière ? Contactez-nous pour un devis.
