The ProcartaPlex Human Akt Pathway Panel, phospho, 8plex, enables simultaneous quantitation of eight phosphorylated proteins included in the Akt cell signaling pathway.
The panel is validated for use with cell lysates. For preparation of cell lysates, we recommend the use of either ProcartaPlex Cell Lysis Buffer (Cat. No. EPX-999999-901) or Cell Extraction Buffer (Cat. No. FNN0011) supplemented with 0.5 M EDTA and Halt Protease and Phosphatase Inhibitor Single-Use Cocktail (100X) (Cat. No. 78440). These buffers are not included with the panel. Depending on your cell system, other buffers can be suitable as well.
The panel is provided in a ready-to-use format with individual vials of capture and detection reagents formulated at 1X concentration, requiring less pipetting and experimental setup.
ProcartaPlex preconfigured panels are extensively tested for analyte combinability, interference, and cross-reactivity to provide the highest level of validation and precision. All ProcartaPlex panels are supplied with the necessary reagents to perform the assay.
This kit is compatible with all Luminex instruments. If you use a xMAP INTELLIFLEX DR-SE instrument, we recommend using our ProcartaPlex Human Akt Pathway Dual Reporter Panel, 2x8plex (Cat.No. EPX080-97200-DR), which enables the simultaneous quantitation of total and phosphorylated proteins on the same bead in a single well.
ProcartaPlex immunoassays are based on the principles of a sandwich ELISA, using two highly specific antibodies binding to different epitopes of one protein to quantitate all protein targets simultaneously using a Luminex instrument. ProcartaPlex multiplex assays require as little as 25 μL of plasma or serum or 50 μL of cell culture supernatant and just four hours to obtain analyzed results.
ProcartaPlex assays utilize Luminex xMAP (multianalyte profiling) technology for the simultaneous detection and quantitation of up to 80 protein targets in a single 25–50 μL sample from plasma, serum, cell culture supernatant, cell lysate, and other bodily fluids.
| Marque | Invitrogen |
|---|---|
| Réf. B-LABO | BL-RE-097532 |
| Réf. fabricant | 18476798 |
| Type de produit | Multiplex Panel |
| Quantité | 96 Tests |
| Espèces | Human |
| Combinability | Non-Combinable |
| Type de microbilles | Magnetic |
| Sensibilité du dosage | See Certificate of Analysis |
| Plage de dosage | See Certificate of Analysis |
| Format | Multiplex Kit |
| À utiliser avec (équipement) | Luminex™ Instruments |
| Expression | Akt |
| Alias de gène | Akt, PKB, Protein kinase B |
| Identification génétique (Entrez) | 207 |
| Symbole de gène(s) | Akt |
| Multiplex, taille Plex n° | 8-plex |
| Gamme de produits | ProcartaPlex™ |
| Type d’échantillon | Cell lysates |
| Volume d’échantillon | Cell lysates (prediluted): 25 μL |
| ID UniProt | P31749 |
| Conditions d’expédition | Wet Ice |
| dimResearchArea | Signaling |
