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Invitrogen™ TrueTag™ Donor DNA Kit, DDK | B-LABO France
Invitrogen™ TrueTag™ Donor DNA Kit, DDK
Réf. : BL-RE-087235Réf. fabricant : 17776559
572,00 € HT
The Invitrogen TrueTag Donor DNA Kit, DDK, provides a rapid solution to drive high-efficiency gene tagging (up to 100% edited cells) with transfection-ready donor DNA for genome editing experiments.
À utiliser avec (application)
Protein Detection and Isolation
Nbre de réactions
10 reactions
Marque
Invitrogen
Points forts
Each kit is designed to enable users to
Tag the carboxy- (C-) or amino- (N-) terminus of the gene of interest
Choose from puromycin or blasticidin selection markers – whatever best suits the cell model
Easily remove antibiotic resistance markers with Cre/Lox following cell enrichment
Included in the kit
Four linear donor templates: N-terminus/puromycin resistance, N-terminus/blasticidin resistance, C-terminus/puromycin resistance, C-terminus/blasticidin resistance
Phusion Flash High-Fidelity PCR Master Mix
PCR cleanup columns and buffers
Positive control primers to tag human beta-actin (ACTB)
Intuitive software to rapidly design gene-specific reagents
Target-specific gene editing reagents are required for proper knock-in of your fluorescent tag. The Invitrogen TrueDesign Genome Editor web-based software does the design work for you:
Identifies target gene transcript
Designates insertion site
Designs and enables easy ordering of all necessary components
Required reagents available via TrueDesign software (not included in kit)
CRISPR guide RNA or TALENs designed adjacent to N- or C-terminus
Oligos to PCR-amplify the donor and add appropriate homology arms
Sequencing primers for assessment of CRISPR-Cas9 cleavage efficiency
Verification primers to validate proper insertion of the TrueTag DNA Donor
Also required and available for purchase separately or as part of TrueDesign workflow
TrueCut Cas9 Protein v2, TrueCut HiFi Cas9 Protein, or GeneART CRISPR mRNA
CRISPRMAX Transfection Reagent or MessengerMAX Transfection Reagent
Caractéristiques principales
A rapid solution to drive high-efficiency gene tagging (up to 100% edited cells) with transfection-ready donor DNA for genome editing experiments
Kit provides everything needed to produce a high-quality DNA donor to tag the gene with a selection marker and DDK
Simply provide two locus-specific primers to add homology arms, PCR-amplify, and purify for a transfection-ready donor in hours, not days
One-step PCR amplification to generate donor templates – no cloning required
Enrich up to 100% DDK-tagged cells
Caractéristiques techniques
Marque
Invitrogen
Réf. B-LABO
BL-RE-087235
Réf. fabricant
17776559
À utiliser avec (application)
Protein Detection and Isolation
Nbre de réactions
10 reactions
Une question, une configuration particulière ? Contactez-nous pour un devis.
