The Invitrogen TrueTag Knockout Enrichment Donor DNA Kit provides a rapid solution to drive high-efficiency gene knockout and enrichment (up to 100% edited cells) with transfection-ready donor DNA for genome editing experiments.
À utiliser avec (application)
Gene Functional Analysis
Quantité
10 reactions
Marque
Invitrogen
Points forts
Each kit is designed to enable users to
Insert 3X STOP codons to knock out gene function
Tag each allele with a different fluorophore (GFP or RFP) to verify bi-allelic knockout
Utilize puromycin and blasticidin selection markers for bi-allelic knockout confirmation
Easily remove antibiotic resistance markers with Cre/Lox following cell enrichment
Included in the kit
Four linear donor templates: GFP/puromycin resistance, GFP/blasticidin resistance, RFP/puromycin resistance, RFP/blasticidin resistance
Phusion Flash High-Fidelity PCR Master Mix
PCR cleanup columns and buffers
Positive control primers for knockout of human HPRT1
Intuitive software to rapidly design gene-specific reagents
Target-specific gene editing reagents are required for proper insertion of the knockout enrichment DNA donor. The Invitrogen TrueDesign Genome Editor web-based software does the design work for you:
Identifies target gene transcript
Selects a pre-designed or custom guide RNA
Generates primers and enables easy ordering of all necessary components
Required reagents available via TrueDesign software (not included in kit)
CRISPR guide RNA or TALENs designed for high-efficiency cleavage
Oligos to PCR-amplify your donor and add appropriate homology arms
Sequencing primers for assessment of CRISPR-Cas9 cleavage efficiency
Verification primers to validate proper insertion of the TrueTag DNA Donor
Also required and available for purchase separately or as part of TrueDesign workflow
TrueCut Cas9 Protein v2, TrueCut HiFi Cas9 Protein, or GeneART CRISPR mRNA
CRISPRMAX Transfection Reagent or MessengerMAX Transfection Reagent
Caractéristiques principales
A rapid solution to drive high-efficiency gene knockout and enrichment (up to 100% edited cells) with transfection-ready donor DNA for genome editing experiments
Kit provides everything needed to achieve functional gene knockout and enrich the edited cells
The donor produced will insert 3X STOP codons, a selection marker, and a fluorescent tag
Simply provide two locus-specific primers to add homology arms and PCR-amplify the donor
Easily design and order all necessary components to generate a knockout cell line
Save up to 3 months with enrichment compared to single cell isolation and characterization
Leverage a selection marker to obtain up to 100% edited cells
Caractéristiques techniques
Marque
Invitrogen
Réf. B-LABO
BL-RE-087291
Réf. fabricant
17764109
À utiliser avec (application)
Gene Functional Analysis
Quantité
10 reactions
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